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Tissue/ cell preparation: AKR/Cum mouse spontaneous T lymphoma cell line TK1.
This antibody has been shown to be useful for studies of the induction of homotypic cellular aggregation and the blockade of binding to cell adhesion molecules.
Our Abpromise guarantee covers the use of ab25329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|Blocking||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
Flow Cytometry analysis of BALB/c mouse splenocytes labeling Integrin alpha 4+beta 7 with ab25329 at 1 μg/106 cells (purple). A Mouse Anti-Rat IgG2a-CY5 was used as the secondary antibody. Grey - Isotype Control, Rat IgG2a-UNLB, followed by Mouse Anti-Rat IgG2a-CY5.
ab25329 has not yet been referenced specifically in any publications.