Anti-Integrin alpha 4+beta 7 antibody [DATK32] (Biotin) (ab25217)


  • Product nameAnti-Integrin alpha 4+beta 7 antibody [DATK32] (Biotin)
    See all Integrin alpha 4+beta 7 primary antibodies
  • Description
    Rat monoclonal [DATK32] to Integrin alpha 4+beta 7 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Blockingmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    AKR/Cum Mouse spontaneous T lymphoma cell line TK1.

  • Epitopeab25217 recognizes a conformational epitope on the heterodimer.
  • Positive control
    • Flow Cyt: Mouse spleen cells and C57Bl/6 bone marrow cells.
  • General notes

    ab25217 could be useful for induction of homotypic cellular aggregation.



Our Abpromise guarantee covers the use of ab25217 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab18445 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration.


  • RelevanceIntegrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.
  • Cellular localizationMembrane; Single pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen CD49D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin beta 7 antibody
    • Integrin beta 7 subunit antibody
    • ITGA4 antibody
    • ITGB7 antibody
    • LPAM1 antibody
    see all

Anti-Integrin alpha 4+beta 7 antibody [DATK32] (Biotin) images

  • C57Bl/6 bone marrow cells were stained with APC-conjugated anti-mouse CD45R (B220) and 0.25µg ab25217 (right) or 0.25µg biotin-conjugated rat IgG2a isotype control (left) followed by streptavidin PE.

References for Anti-Integrin alpha 4+beta 7 antibody [DATK32] (Biotin) (ab25217)

This product has been referenced in:
  • Andrew DP  et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol 153:3847-61 (1994). Read more (PubMed: 7523506) »
  • Hamann A  et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol 152:3282-93 (1994). Read more (PubMed: 7511642) »

See all 2 Publications for this product

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