Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (Allophycocyanin) (ab93539)

Overview

  • Product nameAnti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (Allophycocyanin)
    See all Integrin alpha 4+beta 7 primary antibodies
  • Description
    Rat monoclonal [DATK32 (DATK-32)] to Integrin alpha 4+beta 7 (Allophycocyanin)
  • ConjugationAllophycocyanin. Ex: 645nm, Em: 660nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: AKR/Cum Mouse spontaneous T lymphoma cell line TK1.

  • Positive control
    • Mouse splenocytes and C57Bl/6 bone marrow cells
  • General notes


    Binding of the DATK32 monoclonal antibody has been shown to induce aggregation of the CD8+ T cell lymphoma TK1, and block Integrin alpha 4 beta 7 mediated cell adhesion.

Properties

Applications

Our Abpromise guarantee covers the use of ab93539 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.125-0.5µg for 105-8 cells.

Use 0.125 - 0.5 µg for 105 - 108 cells in a 100 µl volume.

Target

  • RelevanceIntegrin alpha 4 beta 7, also known as, the lymphocyte Peyer's patch adhesion molecule (LPAM1) is a member of the integrin family of cell surface receptors. It is expressed primarily on mucosal lymphocytes, but is also present on NK cells and eosinophils. The alpha 4 beta 7 heterodimer mediates the binding of lymphocytes to its ligand, mucosal vascular addressin (MAdCAM1) on the high endothelial venules, thereby directing the homing of lymphocytes into Peyer's patches and intestinal lamina propria.
  • Cellular localizationMembrane; Single pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen CD49D antibody
    • CD49d antibody
    • IA4 antibody
    • Integrin alpha 4 subunit antibody
    • Integrin alpha IV antibody
    • Integrin beta 7 antibody
    • Integrin beta 7 subunit antibody
    • ITGA4 antibody
    • ITGB7 antibody
    • LPAM1 antibody
    see all

Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (Allophycocyanin) images

  • Staining of C57Bl/6 bone marrow cells with FITC anti-Mouse/Human B220 (RA3-6B2) and 0.125 µg of APC Rat IgG2a Isotype Control (left), or 0.125 µg of ab93539 (right). Total viable cells were used for analysis.

References for Anti-Integrin alpha 4+beta 7 antibody [DATK32 (DATK-32)] (Allophycocyanin) (ab93539)

This product has been referenced in:
  • Andrew DP  et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7-mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J Immunol 153:3847-61 (1994). Flow Cyt . Read more (PubMed: 7523506) »
  • Hamann A  et al. Role of alpha 4-integrins in lymphocyte homing to mucosal tissues in vivo. J Immunol 152:3282-93 (1994). Flow Cyt . Read more (PubMed: 7511642) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"