Anti-Integrin alpha 5 antibody [5H10-27] (Biotin) (ab25189)

Overview

  • Product name
    Anti-Integrin alpha 5 antibody [5H10-27] (Biotin)
    See all Integrin alpha 5 primary antibodies
  • Description
    Rat monoclonal [5H10-27] to Integrin alpha 5 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab25189 recognises alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
  • Tested applications
    Suitable for: Flow Cyt, Blocking, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation of C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)

  • General notes


    The monoclonal antibody 5H10-27 reacts with the alpha 5 chain (VLA-5) of the CD49e/CD29 heterodimeric fibronectin receptor. Soluble 5H10-27 antibody inhibits VLA-5-mediated functions in vitro. Immobilized 5H10-27 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound anti-CD3 monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab25189 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18445-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use at an assay dependent concentration.

In vitro blocking of adhesion.

IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

T-cell co-stimulation.

Target

  • Function
    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Images

References

ab25189 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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