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C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)
Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.
Our Abpromise guarantee covers the use of ab25251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.
T-cell co-stimulation, In vitro blocking of adhesion.
|ICC/IF||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.
Use up to 1ug/106 cells.
ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IHC-Fr||Use at an assay dependent concentration.|