Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (ab25251)

Overview

  • Product nameAnti-Integrin alpha 5 antibody [5H10-27 (MFR5)]
    See all Integrin alpha 5 primary antibodies
  • Description
    Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5
  • Specificityab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor.
  • Tested applicationsSuitable for: Functional Studies, ICC/IF, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C57BL/6 x A/J F1 mast cell line MC/9 (Mouse)

  • General notes


    Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab25251 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

T-cell co-stimulation, In vitro blocking of adhesion.

ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Use up to 1ug/106 cells.





ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] images

  • ab25251 staining Integrin alpha 5 in Mouse Embryonic Fibroblast (MEF) cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde and permeabilized in Triton X-100. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 546-conjugated goat anti-rat polyclonal, diluted 1/200.

    See Abreview

References for Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (ab25251)

This product has been referenced in:
  • Rich S  et al. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol 157:2916-23 (1996). Read more (PubMed: 8816397) »
  • Uhlenkott CE  et al. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis 14:125-37 (1996). Read more (PubMed: 8605726) »

See all 5 Publications for this product

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Thank you for contacting us.

I can confirm that the soluble Anti-Integrin alpha 5 antibody (clone 5H10-27 (MFR5)) was used to inhibit VLA5-mediated functions in vitro.

Since applications vary, we recommend to determine the optimum wor...

Read More
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cell line ( cervical cancer))
Specification Hela cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

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Verified customer

Submitted Dec 01 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

Submitted Nov 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"