Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)] (ab25251)
Key features and details
- Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5
- Suitable for: ICC/IF, IHC-Fr, Flow Cyt, Functional Studies
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-Integrin alpha 5 antibody [5H10-27 (MFR5)]
See all Integrin alpha 5 primary antibodies -
Description
Rat monoclonal [5H10-27 (MFR5)] to Integrin alpha 5 -
Host species
Rat -
Specificity
ab25251 is specific for the alpha 5 subunit of the integrin alpha 5 beta 1 fibronectin receptor. -
Tested applications
Suitable for: ICC/IF, IHC-Fr, Flow Cyt, Functional Studiesmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin alpha 5. C57BL/6 x A/J F1 mouse mast cell line MC/9
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General notes
Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituent: 100% Borate buffered saline -
Concentration information loading...
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Primary antibody notes
Soluble ab25251 inhibits VLA5-mediated functions in vitro. Immobilized ab25251 has also been demonstrated to co-stimulate the proliferative response of CD8+ T cells to plate-bound antiCD3 monoclonal antibody. -
Clonality
Monoclonal -
Clone number
5H10-27 (MFR5) -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25251 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (2) |
Use at an assay dependent concentration.
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IHC-Fr |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
Use up to 1ug/10^6 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
|
Functional Studies |
Use at an assay dependent concentration.
T-cell co-stimulation, In vitro blocking of adhesion. |
Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Use up to 1ug/10^6 cells. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Functional Studies
Use at an assay dependent concentration. T-cell co-stimulation, In vitro blocking of adhesion. |
Target
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Function
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16402 Mouse
- SwissProt: P11688 Mouse
- Unigene: 16234 Mouse
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Alternative names
- CD49 antigen-like family member E antibody
- CD49e antibody
- Fibronectin receptor subunit alpha antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab25251 has been referenced in 9 publications.
- Verstappen JFM et al. RGD-functionalized supported lipid bilayers modulate pre-osteoblast adherence and promote osteogenic differentiation. J Biomed Mater Res A 108:923-937 (2020). PubMed: 31895490
- Ho TC et al. The Psoriasis Therapeutic Potential of a Novel Short Laminin Peptide C16. Int J Mol Sci 20:N/A (2019). PubMed: 31252620
- Wang X et al. Knockdown of HMGN2 increases the internalization of Klebsiella pneumoniae by respiratory epithelial cells through the regulation of a5ß1 integrin expression. Int J Mol Med 38:737-46 (2016). Human . PubMed: 27460641
- Luxenburg C et al. Involvement of actin polymerization in podosome dynamics. J Cell Sci 125:1666-72 (2012). PubMed: 22328507
- Rich S et al. Role of alpha 5 beta 1 integrin in TGF-beta 1-costimulated CD8+ T cell growth and apoptosis. J Immunol 157:2916-23 (1996). PubMed: 8816397
- Uhlenkott CE et al. Attachment, invasion, chemotaxis, and proteinase expression of B16-BL6 melanoma cells exhibiting a low metastatic phenotype after exposure to dietary restriction of tyrosine and phenylalanine. Clin Exp Metastasis 14:125-37 (1996). PubMed: 8605726
- Schultz JF & Armant DR Beta 1- and beta 3-class integrins mediate fibronectin binding activity at the surface of developing mouse peri-implantation blastocysts. Regulation by ligand-induced mobilization of stored receptor. J Biol Chem 270:11522-31 (1995). PubMed: 7538116
- Halvorson MJ & Coligan JE Enhancement of VLA integrin receptor function on thymocytes by cAMP is dependent on the maturation stage of the thymocytes. J Immunol 155:4567-74 (1995). PubMed: 7594454
- Kinashi T & Springer TA Adhesion molecules in hematopoietic cells. Blood Cells 20:25-44 (1994). PubMed: 7527678