The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Flow Cyt: 1/Neat - 1/10. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Note: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.