Anti-Integrin alpha 5 antibody [P1D6] (ab78614)


  • Product name
    Anti-Integrin alpha 5 antibody [P1D6]
    See all Integrin alpha 5 primary antibodies
  • Description
    Mouse monoclonal [P1D6] to Integrin alpha 5
  • Host species
  • Specificity
    ab78614 is not reactive with the denatured Integrin alpha 5 subunit.
  • Tested applications
    Suitable for: Dot blot, IHC-Fr, Flow Cyt, Inhibition Assay, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Integrin alpha 5.
    Database link: P08648

  • Positive control
    • Ovary. HeLa cells. Widely expressed



Our Abpromise guarantee covers the use of ab78614 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab91537 - Mouse monoclonal IgG3, is suitable for use as an isotype control with this antibody.


Inhibition Assay Use at an assay dependent concentration. Suitable for use in attachment inhibition assays using fibroblasts, endothelial cells, platelets and T and B lymphocytes on fibronectin.
ICC/IF 1/200.


  • Function
    Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all


  • Overlay histogram showing HeLa cells stained with ab78614 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine (ab120050) to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab78614, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG3 [MG3-35] (ab18394, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed HeLa cells used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Flow Cytometry Analysis: Representative lot data.
    Cell surface staining of HT-1080 cells using 1 µg/mL of ab78614 (shaded histogram) and an isotype control (unshaded histogram). Cells were detected using a mouse IgG-FITC.



This product has been referenced in:
  • Sun M  et al. Extracellular matrix stiffness controls osteogenic differentiation of mesenchymal stem cells mediated by integrin a5. Stem Cell Res Ther 9:52 (2018). Read more (PubMed: 29490668) »
  • Breuksch I  et al. Integrin a5 triggers the metastatic potential in renal cell carcinoma. Oncotarget 8:107530-107542 (2017). Read more (PubMed: 29296184) »

See all 4 Publications for this product

Customer reviews and Q&As

I would recommend Anti-Integrin alpha 5 antibody [P1D6] (ab78614) which is the only anti-integrin alpha 5 antibody we list in our catalogue whose inhibitory activity has been previously tested. It is therefore suitable for use in attachment inhibi...

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Thank you for your enquiry.

I can confirm that this antibody does not contain azide and the storage buffer consists of 250 mM Sodium chloride, 20mM Sodium phosphate buffer, pH 7.6 which are not toxic. Thus, we do not have MSDS document for th...

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Thank you for your enquiry.

I apologise for the delay in getting back to you. I have been revewing and checking the immunogen sequences of our Integrin alpha 5 antibody. Regrettably, so far I am sorry I have not found one that would match yo...

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Immunocytochemistry/ Immunofluorescence
Human Cell (Human foreskin fibroblast)
Human foreskin fibroblast
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C

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Verified customer

Submitted Oct 25 2011


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