Anti-Integrin alpha 5 antibody [SAM-1] (ab6131)

Overview

  • Product nameAnti-Integrin alpha 5 antibody [SAM-1]
    See all Integrin alpha 5 primary antibodies
  • Description
    Mouse monoclonal [SAM-1] to Integrin alpha 5
  • SpecificityNKI-SAM-1 recognizes the 135-kDa integrin- alpha5 monocyte-specific antigen. As a result NKI-SAM-1 strongly reacts with monocytes, and weakly with granulocytes, platelets and T-lymphocytes. Its exact epitope is not known.
  • Tested applicationsSuitable for: Flow Cyt, IP, ICC/IF, IHC-Frmore details
  • Species reactivity
    Reacts with: Human, Macaque Monkey
  • Immunogen

    Integrin alpha 5 positive cell preparation.

  • General notes

    Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.

    NKI-SAM-1 is a monoclonal antibody obtained by fusion of mouse myeloma cells with spleen cells from BALB/C mice immunized with U937 histiocytic lymphoma cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS (pH 7.4)
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesIntegrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
  • ClonalityMonoclonal
  • Clone numberSAM-1
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab6131 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2-0.5µg for 106 cells.
IP Use a concentration of 0.5 - 2 µg/ml.
ICC/IF 1/200. (see Abreview).
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionIntegrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD49 antigen-like family member E antibody
    • CD49e antibody
    • Fibronectin receptor subunit alpha antibody
    • Fibronectin receptor, alpha subunit antibody
    • FNRA antibody
    • Integrin alpha 5 (fibronectin receptor alpha) antibody
    • Integrin alpha-5 antibody
    • Integrin alpha-5 light chain antibody
    • Integrin alpha-F antibody
    • Integrin, alpha 5 (fibronectin receptor, alpha polypeptide) antibody
    • ITA5_HUMAN antibody
    • Itga5 antibody
    • Very late activation protein 5, alpha subunit antibody
    • VLA-5 antibody
    • VLA5 antibody
    • VLA5A antibody
    see all

Anti-Integrin alpha 5 antibody [SAM-1] images

  • ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary.

    See Abreview

  • ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min.  Gating was done on synovial fibroblasts. The  sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C.  A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used).

    See Abreview

References for Anti-Integrin alpha 5 antibody [SAM-1] (ab6131)

This product has been referenced in:
  • Tartaglia LJ  et al. Molecular cloning, overexpression, and an efficient one-step purification of a5ß1 integrin. Protein Expr Purif 92:21-8 (2013). Read more (PubMed: 23993979) »
  • Rowland TJ  et al. Roles of integrins in human induced pluripotent stem cell growth on Matrigel and vitronectin. Stem Cells Dev 19:1231-40 (2010). ICC/IF ; Human . Read more (PubMed: 19811096) »

See all 2 Publications for this product

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Thank you for your enquiry.

I apologise for the delay in getting back to you. I have been revewing and checking the immunogen sequences of our Integrin alpha 5 antibody. Regrettably, so far I am sorry I have not found one that would match yo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (synovial fibroblasts)
Specification synovial fibroblasts
Fixation Paraformaldehyde
Permeabilization No
Gating Strategy only synovial fibroblasts
Username

Dr. Torsten Lowin

Verified customer

Submitted Aug 13 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Renal Epithelial Cell)
Specification Renal Epithelial Cell
Fixative Methanol
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4%
Username

Abcam user community

Verified customer

Submitted Aug 10 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"