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Integrin alpha 5 positive cell preparation.
Integrin alpha 5 is a heterodimer that associates noncovalently with CD29/integrin beta 1 subunit to form the alpha-5-beta-1 very late antigen (VLA-5) complex. VLA-5 is a fibronectin receptor that is expressed on thymocytes, T-cells, monocytes and platelets. It is also found on very early B-cells and activated B-cells. VLA-5-mediated binding to fibronectin sends a costimulatory signal to T-cells and enhances Fc-gamma-R- and complement receptor-mediated phago-cytosis. It is also involved in monocyte migration into extracellular tissues.
NKI-SAM-1 is a monoclonal antibody obtained by fusion of mouse myeloma cells with spleen cells from BALB/C mice immunized with U937 histiocytic lymphoma cells.
Our Abpromise guarantee covers the use of ab6131 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2-0.5µg for 106 cells.|
|IP||Use a concentration of 0.5 - 2 µg/ml.|
|ICC/IF||1/200. (see Abreview).|
|IHC-Fr||Use at an assay dependent concentration.|
ab6131 at 1/200 staining human retinal epithelial cells by ICC/IF. The cells were permeabilized with 100% methanol and blocked with 1%BSA / 4% goat serum prior to incubation with the antibody for 30 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit IgG (H+L) was used as the secondary.
ab6131 staining Integrin alpha 5 in Human synovial fibroblasts by Flow Cytometry. Cells were detached by accutase, spun and fixed in 1,5 % formalin for 10 min. Gating was done on synovial fibroblasts. The sample was incubated with the primary antibody, dilution 1/500 (1% BSA in PBS), for 20 minutes at 25°C. A PE-conjugated Goat polyclonal to mouse IgG, dilution 1/200, was used as secondary antibody. The flow cytometry plot is an overlay of the isotype control ( IgG2b, red peak) with the sample (white peak); (equal amounts of isotype IgG2b and antibody were used).