Anti-Integrin alpha 5 + beta 1 antibody [P1D6] (ab75472)


  • Product nameAnti-Integrin alpha 5 + beta 1 antibody [P1D6]
  • Description
    Mouse monoclonal [P1D6] to Integrin alpha 5 + beta 1
  • SpecificityThis antibody reacts with the a5 integrin subunit. It neutralizes binding of alpha5 beta1 to the central cell adhesion domain of fibronectin, which is located within a 80kDa tryptic fragment.
  • Tested applicationsSuitable for: IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human HT1080 fibrosarcoma cells



Our Abpromise guarantee covers the use of ab75472 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/1000.
ICC/IF 1/1000.


  • RelevanceIntegrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. Integrin alpha 5 belongs to the integrin alpha chain family. It contains seven FG-GAP repeats. Integrin beta 1 belongs to the integrin beta chain family. It contains one VWFA domain. Integrin alpha-5/beta-1 is a receptor for fibronectin and fibrinogen. It recognizes the sequence R-G-D in its ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Cellular localizationIntegrin alpha 5: Membrane; Single-pass type I membrane protein. Integrin beta 1: Cell membrane; Single-pass type I membrane protein. Melanosome.
  • Database links
  • Alternative names
    • CD49e antibody
    • FNRA antibody
    • FNRB antibody
    • Integrin alpha 5 antibody
    • Integrin beta 1 antibody
    • ITGA5 antibody
    • ITGB1 antibody
    • MDF2 antibody
    • VLA 5 antibody
    • VLA BETA antibody
    • VLA5 antibody
    see all

References for Anti-Integrin alpha 5 + beta 1 antibody [P1D6] (ab75472)

This product has been referenced in:
  • Wang Q  et al. Extracellular calumenin suppresses ERK1/2 signaling and cell migration by protecting fibulin-1 from MMP-13-mediated proteolysis. Oncogene N/A:N/A (2014). Read more (PubMed: 24632605) »
  • Maherally Z  et al. Receptors for hyaluronic acid and poliovirus: a combinatorial role in glioma invasion? PLoS One 7:e30691 (2012). ICC/IF ; Human . Read more (PubMed: 22363471) »

See all 3 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Candida albicans Cell (Hypha and yeast cells)
Permeabilization Yes - Saponin
Specification Hypha and yeast cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative Paraformaldehyde

Angela Lopez

Verified customer

Submitted Jul 28 2016

Thank you for your enquiry.

I have been able to confirm thatab75472 Anti-Integrin alpha 5 + beta 1 antibody [P1D6]is sold as ascites. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will ...

Read More

That is no problem. If you have any further questions, please do not hesitate to contact us.

Until then, I wish you all the best with your research.

Thank you for contacting us yesterday.

I am sorry that the concentration function on the datasheet of Anti-Integrin alpha 5 + beta 1 antibody [P1D6] (ab75472) does not appear to be functioning properly. We will be looking into this. I can inf...

Read More

Thank you for your inquiry.

Please refer to the following reference:

Wayner EA, Carter WG. Identification of multiple cell surface receptors for fibronectin and collagen in human fibrosarcoma cells possessing unique a and common b s...

Read More

Thank you for your call today and for your questions about ab75472.

I have attached the reference that we discussed, and the protocol for adhesion inhibition can be found on page 3 under the small font "Stage Three: Inhibition of Cell Ad...

Read More