Anti-Integrin alpha 6 antibody (ab97760)
Key features and details
- Rabbit polyclonal to Integrin alpha 6
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Integrin alpha 6 antibody
See all Integrin alpha 6 primary antibodies -
Description
Rabbit polyclonal to Integrin alpha 6 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow -
Immunogen
Recombinant fragment, corresponding to amino acids 526-803 of Human Integrin alpha 6 isoform Alpha-6XIA (NP_000201).
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Positive control
- 293T, A431, HeLaS3, HepG2 and Raji cell lysate. H1299 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab97760 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 120 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 120 kDa. |
Target
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Function
Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760). -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney). -
Involvement in disease
Epidermolysis bullosa letalis, with pyloric atresia -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Post-translational
modificationsIsoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
Palmitoylation by DHHC3 enhances stability and cell surface expression. -
Cellular localization
Cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 535043 Cow
- Entrez Gene: 3655 Human
- Entrez Gene: 16403 Mouse
- Omim: 147556 Human
- SwissProt: P23229 Human
- SwissProt: Q61739 Mouse
- Unigene: 133397 Human
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Alternative names
- Alpha6p antibody
- CD49 antigen-like family member F antibody
- CD49f antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab97760 has been referenced in 9 publications.
- Nanba D et al. EGFR-mediated epidermal stem cell motility drives skin regeneration through COL17A1 proteolysis. J Cell Biol 220:N/A (2021). PubMed: 34550317
- Gao Q et al. Functional Passenger-Strand miRNAs in Exosomes Derived from Human Colon Cancer Cells and Their Heterogeneous Paracrine Effects. Int J Biol Sci 16:1044-1058 (2020). PubMed: 32140072
- Gong LB et al. FKBP10 Acts as a New Biomarker for Prognosis and Lymph Node Metastasis of Gastric Cancer by Bioinformatics Analysis and in Vitro Experiments. Onco Targets Ther 13:7399-7409 (2020). PubMed: 32801763
- Iglesia RP et al. Engagement of cellular prion protein with the co-chaperone Hsp70/90 organizing protein regulates the proliferation of glioblastoma stem-like cells. Stem Cell Res Ther 8:76 (2017). PubMed: 28412969
- Loh KH et al. Proteomic Analysis of Unbounded Cellular Compartments: Synaptic Clefts. Cell 166:1295-1307.e21 (2016). PubMed: 27565350
- Zhao F et al. Up-regulation of integrin a6ß4 expression by mitogens involved in dairy cow mammary development. In Vitro Cell Dev Biol Anim 51:287-99 (2015). PubMed: 25319126
- Termini CM et al. The membrane scaffold CD82 regulates cell adhesion by altering a4 integrin stability and molecular density. Mol Biol Cell 25:1560-73 (2014). PubMed: 24623721
- Kinoshita T et al. Tumour-suppressive microRNA-29s inhibit cancer cell migration and invasion by targeting laminin-integrin signalling in head and neck squamous cell carcinoma. Br J Cancer 109:2636-45 (2013). WB ; Human . PubMed: 24091622
- Greciano PG et al. Laminin 511 partners with laminin 332 to mediate directional migration of Madin-Darby canine kidney epithelial cells. Mol Biol Cell 23:121-36 (2012). WB ; Dog . PubMed: 22031290