Overview

  • Product nameAnti-Integrin alpha 6 antibody
    See all Integrin alpha 6 primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha 6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 526-803 of Human Integrin alpha 6 isoform Alpha-6XIA (NP_000201).

  • Positive control
    • 293T, A431, HeLaS3, HepG2 and Raji cell lysate. H1299 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab97760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 120 kDa.

Target

  • FunctionIntegrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • Involvement in diseaseEpidermolysis bullosa letalis, with pyloric atresia
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    modifications
    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • Cellular localizationCell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody images

  • Anti-Integrin alpha 6 antibody (ab97760) at 1/2000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 120 kDa

References for Anti-Integrin alpha 6 antibody (ab97760)

This product has been referenced in:
  • Zhao F  et al. Up-regulation of integrin a6ß4 expression by mitogens involved in dairy cow mammary development. In Vitro Cell Dev Biol Anim 51:287-99 (2015). Read more (PubMed: 25319126) »
  • Termini CM  et al. The membrane scaffold CD82 regulates cell adhesion by altering a4 integrin stability and molecular density. Mol Biol Cell 25:1560-73 (2014). Read more (PubMed: 24623721) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted May 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (MDCK-II epithelial cells)
Loading amount 25 µg
Specification MDCK-II epithelial cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jose Moyano

Verified customer

Submitted Mar 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"