• Product nameAnti-Integrin alpha 6 antibody
    See all Integrin alpha 6 primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha 6
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Dog, Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 526-803 of Human Integrin alpha 6 isoform Alpha-6XIA (NP_000201).

  • Positive control
    • 293T, A431, HeLaS3, HepG2 and Raji cell lysate. H1299 whole cell lysate.



Our Abpromise guarantee covers the use of ab97760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 120 kDa.


  • FunctionIntegrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
  • Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
  • Involvement in diseaseEpidermolysis bullosa letalis, with pyloric atresia
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Post-translational
    Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
    In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
    Palmitoylation by DHHC3 enhances stability and cell surface expression.
  • Cellular localizationCell membrane. Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha6p antibody
    • CD49 antigen-like family member F antibody
    • CD49f antibody
    • Integrin alpha6B antibody
    • Integrin, alpha 6 antibody
    • ITA6_HUMAN antibody
    • ITGA6 antibody
    • ITGA6B antibody
    • Processed integrin alpha-6 antibody
    • VLA 6 antibody
    • VLA-6 antibody
    see all

Anti-Integrin alpha 6 antibody images

  • Anti-Integrin alpha 6 antibody (ab97760) at 1/2000 dilution + H1299 whole cell lysate at 30 µg

    Predicted band size : 120 kDa

References for Anti-Integrin alpha 6 antibody (ab97760)

This product has been referenced in:
  • Zhao F  et al. Up-regulation of integrin a6ß4 expression by mitogens involved in dairy cow mammary development. In Vitro Cell Dev Biol Anim 51:287-99 (2015). Read more (PubMed: 25319126) »
  • Termini CM  et al. The membrane scaffold CD82 regulates cell adhesion by altering a4 integrin stability and molecular density. Mol Biol Cell 25:1560-73 (2014). Read more (PubMed: 24623721) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 11 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (MDCK-II epithelial cells)
Loading amount 25 µg
Specification MDCK-II epithelial cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Jose Moyano

Verified customer

Submitted Mar 23 2011