Overview

  • Product name
    Anti-Integrin alpha 6 antibody [GoH3] (Biotin)
    See all Integrin alpha 6 primary antibodies
  • Description
    Rat monoclonal [GoH3] to Integrin alpha 6 (Biotin)
  • Conjugation
    Biotin
  • Specificity
    ab28080 recognises CD49F (VLA-6 alpha chain)
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human, Cynomolgus monkey
  • Immunogen

    Tissue/ cell preparation (Mouse) BALB/c mouse mammary tumour cells

Properties

Applications

Our Abpromise guarantee covers the use of ab28080 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use a working dilution of neat to 1/5. Use 10µl for 106 cells in 100ul.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Integrin alpha-6/beta-1 is a receptor for laminin on platelets. Integrin alpha-6/beta-4 is a receptor for laminin in epithelial cells and it plays a critical structural role in the hemidesmosome (By similarity). ITGA6:ITGB4 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGA6:ITGB4 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:22351760).
    • Tissue specificity
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoforms containing segment X1 are ubiquitously expressed. Isoforms containing segment X1X2 are expressed in heart, kidney, placenta, colon, duodenum, myoblasts and myotubes, and in a limited number of cell lines; they are always coexpressed with the ubiquitous isoform containing segment X1. In some tissues (e.g. Salivary gland), isoforms containing cytoplasmic segment A and isoforms containing segment B are detected while in others, only isoforms containing one cytoplasmic segment are found (segment A in epidermis and segment B in kidney).
    • Involvement in disease
      Epidermolysis bullosa letalis, with pyloric atresia
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Post-translational
      modifications
      Isoforms containing segment A, but not segment B, are the major targets for PMA-induced phosphorylation. Phosphorylation occurs on 'Ser-1103' of isoform alpha-6X1X2A. Phosphorylation is not required for the induction of integrin alpha-6A/beta-1 high affinity but may reduce the affinity for ligand.
      In invasive prostate cancer ITGA6 undergoes PLAU-mediated cleavage at residues Arg-634-635-Arg in a time-dependent manner enhancing cell invasion and migration in vitro.
      Palmitoylation by DHHC3 enhances stability and cell surface expression.
    • Cellular localization
      Cell membrane. Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Alpha6p antibody
      • CD49 antigen-like family member F antibody
      • CD49f antibody
      • Integrin alpha6B antibody
      • Integrin, alpha 6 antibody
      • ITA6_HUMAN antibody
      • ITGA6 antibody
      • ITGA6B antibody
      • Processed integrin alpha-6 antibody
      • VLA 6 antibody
      • VLA-6 antibody
      see all

    Images

    • ab28080, at 10µl per 106 cells, staining human Integrin alpha 6 in peripheral blood lymphocytes by Flow Cytometry

    References

    This product has been referenced in:
    • Lim E  et al. Aberrant luminal progenitors as the candidate target population for basal tumor development in BRCA1 mutation carriers. Nat Med 15:907-13 (2009). Read more (PubMed: 19648928) »
    • Yoshino N  et al. Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp Anim 49:97-110 (2000). Read more (PubMed: 10889948) »

    See all 3 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab28080.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up