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Cell preparation: SW1222 human colorectal cell line.
Our Abpromise guarantee covers the use of ab20142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. (from PubMed:17370299)|
|IHC-Fr||Use at an assay dependent concentration.|
|Flow Cyt||Use 1µg for 106 cells. (paraformaldehyde or methanol fixed cells)
ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of
Overlay histogram showing HT1080 cells stained with
Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.