Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 115 kDa).

Target

  • Function
    Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin.
  • Tissue specificity
    In airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes. Abundantly expressed in fetal lung and lung cancers.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha RLC antibody
    • Integrin alpha 4 like antibody
    • Integrin alpha RLC antibody
    • Integrin alpha-9 antibody
    • Integrin alpha-RLC antibody
    • ITA9_HUMAN antibody
    • ITGA 4L antibody
    • ITGA 9 antibody
    • ITGA4L antibody
    • ITGA9 antibody
    • RLC antibody
    see all

Images

  • All lanes : Anti-Integrin alpha 9 antibody (ab87995) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate - Fetal Tissue
    Lane 2 : Human liver tissue lysate - total protein (ab29889)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 115 kDa
    Observed band size : 145 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References

This product has been referenced in:
  • Chen WS  et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun 7:11302 (2016). Read more (PubMed: 27066737) »
  • Yamaguchi Y  et al. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage. J Biol Chem 288:3097-111 (2013). WB ; Human . Read more (PubMed: 23204518) »

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab87995.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up