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Rabbit polyclonal to Integrin alpha 9
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Integrin alpha 9.
(Peptide available as
This antibody gave a positive signal in Human Fetal Lung and Adult Liver Tissue lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 145 kDa (predicted molecular weight: 115 kDa).
Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin.
In airway epithelium, in the basal layer of squamous epithelium, and in smooth muscle, skeletal muscle, and hepatocytes. Abundantly expressed in fetal lung and lung cancers.
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Information by UniProt
Alpha RLC antibody
Integrin alpha 4 like antibody
Integrin alpha RLC antibody
Western blot - Integrin alpha 9 antibody (ab87995)
All lanes :
Anti-Integrin alpha 9 antibody (ab87995) at 1 µg/ml
Lane 1 :
Lung (Human) Tissue Lysate - Fetal Tissue
Lane 2 :
Human liver tissue lysate - total protein (
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
145 kDa (
why is the actual band size different from the predicted?
Integrin alpha 9 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This product has been referenced in:
et al. Pathological lymphangiogenesis is modulated by galectin-8-dependent crosstalk between podoplanin and integrin-associated VEGFR-3. Nat Commun 7:11302 (2016).
Read more (PubMed: 27066737) »
et al. Thrombin-cleaved fragments of osteopontin are overexpressed in malignant glial tumors and provide a molecular niche with survival advantage. J Biol Chem 288:3097-111 (2013).
Read more (PubMed: 23204518) »
See all 2 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"