Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947)

Overview

  • Product name
    Anti-Integrin alpha 9+beta 1 antibody [Y9A2]
  • Description
    Mouse monoclonal [Y9A2] to Integrin alpha 9+beta 1
  • Specificity
    Ab27947 recognises alpha9 integrin expressed as a heterodimer in association with the beta1 integrin.
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WB, ICCmore details
    Unsuitable for: IHC-FoFr,IHC-P or IHC-R
  • Species reactivity
    Reacts with: Rat, Guinea pig, Human
  • Immunogen

    Tissue/ cell preparation (Human): alpha9 integrin transfected lambda cells.

  • General notes


    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide.
    Constituents: PBS, pH 7.4.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab27947 was purified by affinity chromatography, from tissue culture supernatant.
  • Primary antibody notes
    This clone (Y9A2) inhibits adhesion of alpha9 expressing cells to N terminal osteopontin.
  • Clonality
    Monoclonal
  • Clone number
    Y9A2
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27947 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20810886
Flow Cyt Use 10µl for 106 cells.

(in 100µl). ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. PubMed: 20810886
ICC Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IHC-FoFr,IHC-P or IHC-R.
  • Target

    • Relevance
      Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Integrin alpha 9, when bound to the integrin beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of integrin alpha 9 has been found to be upregulated in small cell lung cancers.
    • Cellular localization
      Type I membrane protein.
    • Database links
    • Alternative names
      • CD29 antibody
      • FNRB antibody
      • GPIIA antibody
      • ITGA9 antibody
      • ITGB1 antibody
      • MDF2 antibody
      • MSK12 antibody
      • VLAB antibody
      see all

    References for Anti-Integrin alpha 9+beta 1 antibody [Y9A2] (ab27947)

    This product has been referenced in:
    • Ou J  et al. Fibronectin extra domain A (EDA) sustains CD133(+)/CD44(+) subpopulation of colorectal cancer cells. Stem Cell Res 11:820-33 (2013). Read more (PubMed: 23811539) »
    • Eva R  et al. Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells. J Neurosci 30:11654-69 (2010). ICC/IF, Inhibition Assay ; Rat . Read more (PubMed: 20810886) »

    See all 5 Publications for this product

    Product Wall

    Thank you for your enquiry.

    The following information is taken from Human Protein Atlas.

    1) Integrin alpha 9 positive control: Digestive tract
    http://www.proteinatlas.org/ENSG00000144668

    2) Integrin beta 1 positive...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cultured Cells (Endothelial cells and fibroblasts cultured from hu)
    Specification
    Endothelial cells and fibroblasts cultured from hu
    Fixative
    Methanol
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 16 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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