Anti-CD103 antibody [2E7] (ab25198)
Key features and details
- Armenian hamster monoclonal [2E7] to CD103
- Suitable for: IHC-Fr, IP, Cellular Activation, Flow Cyt
- Reacts with: Mouse
- Isotype: IgG
Related conjugates and formulations
Overview
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Product name
Anti-CD103 antibody [2E7]
See all CD103 primary antibodies -
Description
Armenian hamster monoclonal [2E7] to CD103 -
Host species
Armenian hamster -
Tested applications
Suitable for: IHC-Fr, IP, Cellular Activation, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to CD103. C57BL/6J mouse intestinal intraepithelial lymphocytes (IEL)
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General notes
This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.20
Constituents: 0.95% Sodium borate, 0.6% Boric Acid -
Concentration information loading...
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Purity
Affinity purified -
Primary antibody notes
This antibody is reported to have signal inducing activity in a redirected lysis assay and to co stimulate IEL and CD8+ lymph node cells in conjunction with anti TCR in vitro, which runs counter to the usual inhibition of CTL by anti integrins. The property of costimulation is not unique to the mouse since the combination of anti human alpha IEL (intraepithelial lymphocytes) plus anti TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated. -
Clonality
Monoclonal -
Clone number
2E7 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25198 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Cellular Activation |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Cellular Activation
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab18479 - Armenian Hamster monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers. -
Tissue specificity
Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16407 Mouse
- SwissProt: Q60677 Mouse
- Unigene: 96 Mouse
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Alternative names
- A530055J10 antibody
- alpha-E1 antibody
- alpha-M290 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25198 has been referenced in 1 publication.
- Zhou J et al. Prognostic and therapeutic value of CD103+ cells in renal cell carcinoma. Exp Ther Med 15:4979-4986 (2018). PubMed: 29805521