Anti-Integrin alpha E antibody [2E7] (Phycoerythrin) (ab25517)

Overview

  • Product name
    Anti-Integrin alpha E antibody [2E7] (Phycoerythrin)
    See all Integrin alpha E primary antibodies
  • Description
    Armenian Hamster monoclonal [2E7] to Integrin alpha E (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab25517 recognises the CD103/alpha IELbeta 7 integrin complex (MW 160 and 115 kDa peptides).
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, IP, Cellular Activationmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes


    The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. This co-stimulatory activity is also shared by the mAb M290. The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilising agent
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. This co-stimulatory activity is also shared by the mAb M290. The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.
  • Clonality
    Monoclonal
  • Clone number
    2E7
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution.
IP Use at an assay dependent dilution.
Cellular Activation Use at an assay dependent dilution.

Target

  • Function
    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • Tissue specificity
    Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A530055J10 antibody
    • alpha-E1 antibody
    • alpha-M290 antibody
    • aM290 antibody
    • Antigen CD103 antibody
    • CD 103 antibody
    • CD103 antibody
    • CD103 antigen antibody
    • HML 1 antigen antibody
    • HML-1 antigen antibody
    • HML1 antigen antibody
    • Human mucosal lymphocyte antigen 1 antibody
    • Human mucosal lymphocyte antigen 1, alpha subunit antibody
    • Huminae antibody
    • Integrin alpha E heavy chain antibody
    • Integrin alpha E light chain antibody
    • Integrin alpha E, epithelial-associated antibody
    • Integrin alpha E1 antibody
    • Integrin alpha IEL antibody
    • Integrin alpha M290 antibody
    • Integrin alpha-E heavy chain antibody
    • Integrin alpha-IEL antibody
    • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
    • Integrin, alpha E antibody
    • ITAE_HUMAN antibody
    • Itgae antibody
    • MGC141996 antibody
    • MGC183601 antibody
    • Mucosal lymphocyte 1 antigen antibody
    • Mucosal lymphocyte antigen 1 antibody
    see all

Images

  • Flow cytometry analysis staining Integrin alpha E in mouse mesenteric lymph node cells using ab25517 at a dilution of 0.1 ug/106 cells.

References

ab25517 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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