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The details of the immunogen for this antibody are not available.
The 2E7 monoclonal antibody exhibits antigen immunoprecipitation patterns similar if not identical to the rat anti-mouse CD103 mAb M290. The 2E7 mAb is reported to have signal-inducing activity in a redirected lysis assay and to co-stimulate IEL and CD8+ lymph node cells in conjunction with anti-TCR in vitro, which runs counter to the usual inhibition of CTL by anti-integrins. This co-stimulatory activity is also shared by the mAb M290. The property of costimulation is not unique to the mouse since the combination of anti-human alpha IEL plus anti-TCR will produce similar proliferation in human cells, with the exception that CD8+ cells are not stimulated.
Our Abpromise guarantee covers the use of ab25517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution.|
|Celluar Activation||Use at an assay dependent dilution.|
Flow cytometry analysis staining Integrin alpha E in mouse mesenteric lymph node cells using ab25517 at a dilution of 0.1 ug/106 cells.
ab25517 has not yet been referenced specifically in any publications.