Anti-Integrin alpha E antibody [2G5.1] (ab19728)

Overview

  • Product nameAnti-Integrin alpha E antibody [2G5.1]
    See all Integrin alpha E primary antibodies
  • Description
    Mouse monoclonal [2G5.1] to Integrin alpha E
  • Tested applicationsSuitable for: IP, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human intestinal lymphocyte (HML1)

Properties

Applications

Our Abpromise guarantee covers the use of ab19728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 105 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use a concentration of 10 - 20 µg/ml.

Target

  • FunctionIntegrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • Tissue specificityExpressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • DomainThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A530055J10 antibody
    • alpha-E1 antibody
    • alpha-M290 antibody
    • aM290 antibody
    • Antigen CD103 antibody
    • CD 103 antibody
    • CD103 antibody
    • CD103 antigen antibody
    • HML 1 antigen antibody
    • HML-1 antigen antibody
    • HML1 antigen antibody
    • Human mucosal lymphocyte antigen 1 antibody
    • Human mucosal lymphocyte antigen 1, alpha subunit antibody
    • Huminae antibody
    • Integrin alpha E heavy chain antibody
    • Integrin alpha E light chain antibody
    • Integrin alpha E, epithelial-associated antibody
    • Integrin alpha E1 antibody
    • Integrin alpha IEL antibody
    • Integrin alpha M290 antibody
    • Integrin alpha-E heavy chain antibody
    • Integrin alpha-IEL antibody
    • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
    • Integrin, alpha E antibody
    • ITAE_HUMAN antibody
    • Itgae antibody
    • MGC141996 antibody
    • MGC183601 antibody
    • Mucosal lymphocyte 1 antigen antibody
    • Mucosal lymphocyte antigen 1 antibody
    see all

References for Anti-Integrin alpha E antibody [2G5.1] (ab19728)

ab19728 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Jejunum)
Specification Jejunum
Fixative Paraformaldehyde
Antigen retrieval step Other
Permeabilization No
Blocking step BSA in FCS (20ml) + DMEM (80ml) as blocking agent for 30 minute(s) · Concentration: 10µg/mL · Temperature: 21°C
Username

Dr. James Jupp

Verified customer

Submitted Nov 01 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"