Overview

  • Product name
    Anti-Integrin alpha E antibody
    See all Integrin alpha E primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha E
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Integrin alpha E.

  • Positive control
    • This antibody gave a positive signal in the following lysates: Jurkat Whole Cell; A431 Whole Cell; HeLa Whole Cell; K562 Whole Cell; Human Small Intestine Tissue; Human Colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab93848 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/250. Detects a band of approximately 120 kDa (predicted molecular weight: 130 kDa).

Target

  • Function
    Integrin alpha-E/beta-7 is a receptor for E-cadherin. It mediates adhesion of intra-epithelial T-lymphocytes to epithelial cell monolayers.
  • Tissue specificity
    Expressed on a subclass of T-lymphocytes known as intra-epithelial lymphocytes which are located between mucosal epithelial cells.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain
    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A530055J10 antibody
    • alpha-E1 antibody
    • alpha-M290 antibody
    • aM290 antibody
    • Antigen CD103 antibody
    • CD 103 antibody
    • CD103 antibody
    • CD103 antigen antibody
    • HML 1 antigen antibody
    • HML-1 antigen antibody
    • HML1 antigen antibody
    • Human mucosal lymphocyte antigen 1 antibody
    • Human mucosal lymphocyte antigen 1, alpha subunit antibody
    • Huminae antibody
    • Integrin alpha E heavy chain antibody
    • Integrin alpha E light chain antibody
    • Integrin alpha E, epithelial-associated antibody
    • Integrin alpha E1 antibody
    • Integrin alpha IEL antibody
    • Integrin alpha M290 antibody
    • Integrin alpha-E heavy chain antibody
    • Integrin alpha-IEL antibody
    • Integrin, alpha E (antigen CD103, human mucosal lymphocyte antigen 1; alpha polypeptide) antibody
    • Integrin, alpha E antibody
    • ITAE_HUMAN antibody
    • Itgae antibody
    • MGC141996 antibody
    • MGC183601 antibody
    • Mucosal lymphocyte 1 antigen antibody
    • Mucosal lymphocyte antigen 1 antibody
    see all

Images

  • All lanes : Anti-Integrin alpha E antibody (ab93848) at 1/250 dilution

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
    Lane 5 : Human small intestine tissue lysate - total protein (ab29276)
    Lane 6 : Human colon tissue lysate - total protein (ab30051)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 130 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
  • ICC/IF image of ab93848 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93848, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and MCF7 cells at 5µg/ml.

References

ab93848 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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