Anti-Integrin alpha V antibody [272-17E6] (ab16821)

Overview

  • Product nameAnti-Integrin alpha V antibody [272-17E6]
    See all Integrin alpha V primary antibodies
  • Description
    Mouse monoclonal [272-17E6] to Integrin alpha V
  • SpecificityCross reacts with all alpha V integrins.
  • Tested applicationsSuitable for: Inhibition Assay, ICC/IF, Blocking, Flow Cyt, ELISA, IPmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse
  • Immunogen

    Full length native protein (purified) (Human).

  • Positive control
    • Human breast carcinoma

Properties

Applications

Our Abpromise guarantee covers the use of ab16821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Blocking Use at an assay dependent concentration. PubMed: 19115199
Flow Cyt Use a concentration of 2.5 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration.
IP Use a concentration of 2.5 µg/ml.

Target

  • FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • integrin alpha V beta 3 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Anti-Integrin alpha V antibody [272-17E6] images

  • ab16821 staining Integrin alpha V in Human prostate cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 1% serum for 2 hours at room temperature. Samples were incubated with primary antibody (1/250 in 1% BSA/PBS) for 15 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/500) was used as secondary antibody.

    See Abreview

  • ab16821 staining Integrin alpha V in Human melanoma cells by Flow Cytometery. Samples were prepared by trypsinizing cells, treatment with STI and spun out trypsin, kept on ice during experiment duration. FSC and SSC were used to gate healthy cell population, which excluded PI positive cells. The primary antibody was diluted 1/250 and incubated with sample for 1 hour at 4°C in 2% FCS/0.1%NaAzide/PBS. An Alexa Fuor® 488-conjugated Goat polyclonal to mouse IgG was used at dilution at 1/500 as secondary antibody.

    See Abreview

References for Anti-Integrin alpha V antibody [272-17E6] (ab16821)

This product has been referenced in:
  • Guye P  et al. Genetically engineering self-organization of human pluripotent stem cells into a liver bud-like tissue using Gata6. Nat Commun 7:10243 (2016). ICC/IF ; Human . Read more (PubMed: 26732624) »
  • Baldwin AK  et al. Epithelial-mesenchymal status influences how cells deposit fibrillin microfibrils. J Cell Sci 127:158-71 (2014). Blocking ; Human . Read more (PubMed: 24190885) »

See all 11 Publications for this product

Product Wall

Vielen Dank für Ihre Anfrage.

Das Labor, von welchem wir diesen Antikörper beziehen, hat uns tatsächlich informiert, dass dieser Antikörper nicht auf Paraffinschnitten funktionieren sollte. Dies ist eine Information von de...

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The exact target epitope was not characterized in-house. Please see the references listed above for more details.
We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse sa...

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I am sorry to confirm that we don't have any different lots in stock at the moment. I would like to reassure you that we monitor feedback closely on a weekly basis and we are not currently concerned about the general quality of this antibody or this ba...

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I am sorry about the issues you have been having. Thank you for bringing this to our attention.

I would very much like to help you with this. However to do that I need the particulars of your protocol. This information is important not only ...

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Thank you very much for contacting us - and sending the enquiry as a table. That clear overview was very helpful :-) As you may know, we have not tested the individual samples for their expression of the mentioned targets. However, according to the ...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Mouse Embryonic Fibroblast (MEF))
Specification Mouse Embryonic Fibroblast (MEF)
Fixative Paraformaldehyde
Permeabilization Yes - triton
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Abcam user community

Verified customer

Submitted Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa cell line ( cervical cancer))
Specification HeLa cell line ( cervical cancer)
Fixative Paraformaldehyde
Permeabilization Yes - triton
Username

Abcam user community

Verified customer

Submitted Aug 24 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Flow Cytometry
Sample Human Cell (melanoma cell line)
Specification melanoma cell line
Preparation Cell harvesting/tissue preparation method: cell line - trypsinized cells, treated with STI and spun out trypsin, kept on ice during experiment duration
Sample buffer: 2% FCS/0.1%NaAzide/PBS
Fixation none
Permeabilization No
Gating Strategy FSC and SSC for healthy cell population, excluded PI positive cells
Username

Abcam user community

Verified customer

Submitted Oct 15 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (prostate)
Specification prostate
Fixative Paraformaldehyde
Permeabilization Yes - 0.2% Triton-X
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Oct 13 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"