Anti-Integrin alpha V antibody (ab112487)

Overview

  • Product nameAnti-Integrin alpha V antibody
    See all Integrin alpha V primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha V
  • Tested applicationsSuitable for: WB, IHC-P, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Integrin alpha V (C terminal).

Properties

Applications

Our Abpromise guarantee covers the use of ab112487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 116 kDa.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionThe alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similaritiesBelongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • integrin alpha V beta 3 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Anti-Integrin alpha V antibody images

  • All lanes : Anti-Integrin alpha V antibody (ab112487)

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat pancreas tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat liver tissue lysate
    Lane 5 : MM231 whole cell lysate
    Lane 6 : HeLa whole cell lysate
    Lane 7 : CEM whole cell lysate
    Lane 8 : Colo320 whole cell lysate


    Predicted band size : 116 kDa
    Observed band size : 116 kDa
  • ab112493 staining CD51 in breast cancer by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Detection by ABC/DAB staining.

References for Anti-Integrin alpha V antibody (ab112487)

ab112487 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted May 11 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"