Overview

  • Product name
    Anti-Integrin alpha V antibody
    See all Integrin alpha V primary antibodies
  • Description
    Rabbit polyclonal to Integrin alpha V
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Sheep, Cow, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human Integrin alpha V.

    (Peptide available as ab94839.)

  • Positive control
    • This antibody gave a positive signal in HUVEC whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab94704 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 130 & 148 kDa (predicted molecular weight: 116 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Sequence similarities
      Belongs to the integrin alpha chain family.
      Contains 7 FG-GAP repeats.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD51 antibody
      • DKFZp686A08142 antibody
      • Integrin alpha five antibody
      • integrin alpha V beta 3 antibody
      • Integrin alpha-5 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • integrin alphaVbeta3 antibody
      • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • MSK 8 antibody
      • Msk8 antibody
      • Vitronectin receptor subunit alpha antibody
      • VNRA antibody
      • VTNR antibody
      see all

    Images

    • Anti-Integrin alpha V antibody (ab94704) at 1 µg/ml + HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 116 kDa
      Observed band size : 130 + 148 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 25 kDa,58 kDa,62 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 10 minutes

    References

    This product has been referenced in:
    • Pijuan-Galitó S  et al. Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture. Nat Commun 7:12170 (2016). Human . Read more (PubMed: 27405751) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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