Recombinant
RabMAb

Anti-Integrin alpha V antibody [EPR16800] (ab179475)

Overview

  • Product name
    Anti-Integrin alpha V antibody [EPR16800]
    See all Integrin alpha V primary antibodies
  • Description
    Rabbit monoclonal [EPR16800] to Integrin alpha V
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Integrin alpha V aa 1-250. The exact sequence is proprietary.
    Database link: P06756

  • Positive control
    • WB: HUVEC, HT-29, A549, C6 and NIH/3T3 whole cell lysates; Human fetal kidney and fetal brain lysates; Mouse brain, kidney and spleen lysates; Rat brain and kidney lysates. IHC: Human kidney, human transitional cell carcinoma of bladder and mouse kidney tissues. ICC/IF: A549 cells. IP: A549 whole cell extract.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab179475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 125, 135 kDa (predicted molecular weight: 116 kDa).
IP 1/40.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Sequence similarities
    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • antigen identified by monoclonal antibody L230 antibody
    • CD 51 antibody
    • CD51 antibody
    • DKFZp686A08142 antibody
    • Integrin alpha five antibody
    • integrin alpha V beta 3 antibody
    • Integrin alpha-5 antibody
    • integrin alpha-V antibody
    • Integrin alpha-V light chain antibody
    • integrin alphaVbeta3 antibody
    • integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
    • ITAV_HUMAN antibody
    • ITGAV antibody
    • MSK 8 antibody
    • Msk8 antibody
    • Vitronectin receptor subunit alpha antibody
    • VNRA antibody
    • VTNR antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on human kidney tubules is observed. Counter stained with Hematoxylin.
    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded human transitional cell carcinoma of bladder tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary antibody at 1/500 dilution. Membrane and weak cytoplasm staining on human transitional cell carcinoma of bladder is observed. Counter stained with Hematoxylin.
    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling Integrin alpha V with ab179475 at 1/500 dilution followed by Goat Anti-Rabbit HRP (IgG H&L) (ab97051) secondary at 1/500 dilution. Membrane and cytoplasm staining on mouse kidney tubule and glomerulus is observed. Counter stained with Hematoxylin.
    Negative control: Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunofluorescent analysis of 100% methanol-fixed, 0.1% Triton X-100 permeabilized A549 (Human lung carcinoma) cells labeling Integrin alpha V with ab179475 at 1/500 dilution, followed by Goat anti-rabbit Alexa Fluor® 488 (IgG) (ab150077) secondary antibody at 1/400 dilution (green). Confocal image showing membrane and cytoplasm staining on A549 cell line. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (goat anti-mouse AlexaFluor®594 secondary antibody) at 1/500 dilution (red).

    The negative controls are as follows:

    -ve control 1: ab179475 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.

    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/50000 dilution

    Lane 1 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysates
    Lane 2 : HT-29 (Human colorectal adenocarcinoma cells) whole cell lysates
    Lane 3 : A549 (Human lung carcinoma) whole cell lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysates
    Lane 2 : Human fetal brain lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • All lanes : Anti-Integrin alpha V antibody [EPR16800] (ab179475) at 1/5000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Mouse kidney lysates
    Lane 3 : Mouse spleen lysates
    Lane 4 : Rat brain lysates
    Lane 5 : Rat kidney lysates
    Lane 6 : C6 (Rat glial tumor cells) whole cell lysates
    Lane 7 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates
    Lane 8 : Raji (Human Burkitt's lymphoma cell line) whole cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 116 kDa
    Observed band size: 125,135 kDa (why is the actual band size different from the predicted?)



    Blocking/dilution buffer: 5% NFDM/TBST.

     

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

  • Integrin alpha V was immunoprecipitated from 1 mg of A549 (Human lung carcinoma) whole cell extract with ab179475 at 1/40 dilution. Western blot analysis was performed from the immunoprecipitate using ab179475 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: A549 whole cell extract.

    Lane 2: PBS instead of A549 whole cell extract.


    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    ab179475 can recognize 135kDa full length Integrin alpha V and 125kDa heavy chain. The 125 kDa band is Integrin alpha V heavy chain.

References

This product has been referenced in:
  • Lagunas AM  et al. Telomere DNA damage signaling regulates cancer stem cell evolution, epithelial mesenchymal transition, and metastasis. Oncotarget 8:80139-80155 (2017). Read more (PubMed: 29113290) »
  • Jiang Y  et al. Abituzumab Targeting of aV-Class Integrins Inhibits Prostate Cancer Progression. Mol Cancer Res 15:875-883 (2017). Read more (PubMed: 28314844) »

See all 7 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse skin)
Gel Running Conditions
Reduced Denaturing (8%)
Loading amount
40 µg
Specification
Mouse skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Mar 20 2018

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