Anti-Integrin alpha V beta 3 antibody [BV3] (ab7166)

Overview

  • Product name
    Anti-Integrin alpha V beta 3 antibody [BV3]
    See all Integrin alpha V beta 3 primary antibodies
  • Description
    Mouse monoclonal [BV3] to Integrin alpha V beta 3
  • Specificity
    This monoclonal antibody binds to human CD51 and Cd61 integrins present on human endothelium cells.
  • Tested applications
    Suitable for: ICC/IF, IHC-P, IHC-Fr, IP, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat, Chicken, Human

Properties

Applications

Our Abpromise guarantee covers the use of ab7166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/250.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      Function: The alpha-V (ITGAV) integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. ITGAV:ITGB3 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415). ITGAV:ITGB3 binds to NRG1 (via EGF domain) and this binding is essential for NRG1-ERBB signaling (PubMed:20682778). ITGAV:ITGB3 binds to FGF1 and this binding is essential for FGF1 signaling (PubMed:18441324). ITGAV:ITGB3 binds to IGF1 and this binding is essential for IGF1 signaling (PubMed:19578119). ITGAV:ITGB3 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGAV:ITGB3 and ITGAV:ITGB6 act as a receptor for fibrillin-1 (FBN1) and mediate R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881). (Microbial infection) Integrin ITGAV:ITGB5 acts as a receptor for adenovirus type C (PubMed:20615244). Integrin ITGAV:ITGB5 and ITGAV:ITGB3 act as receptors for coxsackievirus A9 and B1 (PubMed:9426447, PubMed:15194773, PubMed:7519807). Integrin ITGAV:ITGB3 acts as a receptor for herpes virus 8/HHV-8 (PubMed:18045938). Integrin ITGAV:ITGB6 acts as a receptor for herpes simplex 1/HHV-1 (PubMed:24367260). Integrin ITGAV:ITGB3 acts as a receptor for Human parechovirus 1 (PubMed:11160695). Integrin ITGAV:ITGB3 acts as a receptor for West nile virus (PubMed:23658209). In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). Similarity: Belongs to the integrin alpha chain family. Contains 7 FG-GAP repeats.
    • Cellular localization
      Cell Membrane. Cytoplasmic. Integrin complex. Focal adhesion.
    • Database links
    • Alternative names
      • Antigen identified by monoclonal antibody L230 antibody
      • CD 51 antibody
      • CD 61 antibody
      • CD51 antibody
      • CD51 antigen antibody
      • CD61 antibody
      • CD61 antigen antibody
      • DKFZp686A08142 antibody
      • GP3A antibody
      • GPIIIa antibody
      • Integrin alpha V antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antibody
      • Integrin alpha V vitronectin receptor alpha polypeptide antigen CD51 antibody
      • integrin alpha-V antibody
      • Integrin alpha-V light chain antibody
      • Integrin beta 3 antibody
      • Integrin beta 3 platelet glycoprotein IIIa antigen CD61 antibody
      • Integrin beta chain beta 3 antibody
      • integrinVbeta3 antibody
      • ITAV_HUMAN antibody
      • ITGAV antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • MSK 8 antibody
      • MSK8 antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • Vitronectin receptor alpha polypeptide antibody
      • vitronectin receptor antibody
      • Vitronectin receptor subunit alpha antibody
      • VNR 1 antibody
      • VNR1 antibody
      • VNRA antibody
      see all

    Images

    • ab7166 staining human breast cancer cells by ICC/IF. Cells were paraformaldehyde fixed for 10 minutes and blocked in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/250 and incubated with the sample for 16 hours. An Alexa Fluor® 488 conjugated goat polyclonal to mouse antibody, diluted 1/200 was used as the secondary.

      See Abreview

    • ab7166 staining Integrin alpha V beta 3 in human MeWo melanoma cells by Flow Cytometery. Cells were cultured in 24 well plates and harvested with 100 µL trypsin/ EDTA. Cells were fixed in 1:1 mixture of FACS/Flow and 4% paraformaldehyde in PBS. The primary antibody was diluted to 1 µg/ml and incubated with sample in 3% bovine serum albumin in phosphate buffer for 30 minutes at 37°C. An ab6785, FITC-conjugated goat polyclonal to mouse IgG (1/100) was used as secondary. A459 cells were used as negative control and showed no significant binding of the antibody. Different concentrations were evaluated and 1 µg/ml yield gave the best results. Blank cells were not treated with any antibodies at all, sec only cells were treated with secondary but not primary antibody.

      See Abreview

    References

    This product has been referenced in:
    • Lee MS  et al. Identification of Angiogenesis Rich-Viable Myocardium using RGD Dimer based SPECT after Myocardial Infarction. Sci Rep 6:27520 (2016). IHC . Read more (PubMed: 27283041) »
    • Park SH  et al. RGD Peptide Cell-Surface Display Enhances the Targeting and Therapeutic Efficacy of Attenuated Salmonella-mediated Cancer Therapy. Theranostics 6:1672-82 (2016). IF . Read more (PubMed: 27446500) »

    See all 11 Publications for this product

    Customer reviews and Q&As

    Thank you for your enquiry.

    I can confirm that we recommend not to dilute antibodies before storage unless is states to do so on the individual datasheet. For all antibodies, we suggest to always follow instructions provided on the individua...

    Read More

    Thank you for contacting us.

    Unfortunately, we have not mapped the epitope of ab7166 so we haveno informationif ab7166 is against the entire complex or not.


    I hope this information is helpful to you. Please do not hesitate to ...

    Read More

    Thank you for your email.

    The sensitivity of staining could be increased by;

    - using enhancers; we have DAB enhancer in catalogue, please check the datasheet http://www.abcam.com/DAB-Enhancer-ab675.html
    - Using lower dilution ...

    Read More

    Thank you for your email.

    The CD51 and CD61 target is expressed by endothelial cells, megakaryocytes, monocytes, macrophages and Neutophils which is why the staining is more in the blood vessels.

    It seems the antibody is binding to ...

    Read More

    Thank you for contacting us.

    As discussed on phone please attach images and send it to us.

    We will look forward to hearing from you soon.

    Thank you for your message.

    Yes, I can confirm that this Abreview has been published. Please thank the customer for their valuable feedback.

    You can now use the discount code when the customer places an order for their next primar...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (breast cancer cell line stably expressing Integrin)
    Loading amount
    60 µg
    Specification
    breast cancer cell line stably expressing Integrin
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    Submitted Jan 30 2012

    Thank you for contacting Abcam. I have checked our notes and it appears that ab7166 is unsuitable for western blot, while ab24694 will most probably work for western blotting, its just that we have not tested it in that application. Please let me know ...

    Read More

    Thank you for your enquiry. I would advise you to follow the storage instructions provided on the datahseet. Each product has specific instruction for shipping and storage. As our Abpromise indicates, in the event that a product is not functioning ...

    Read More

    Thank you for contacting us. I have looked at a number of candidates for cell surface markers for HeLa cells, but I am not aware of any that are unique to HeLa cells, if you are trying to separate them from a mixed population. The following pap...

    Read More

    1-10 of 20 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up