Anti-Integrin alpha V + beta 6 antibody [10D5] (ab77906)

Overview

  • Product name
    Anti-Integrin alpha V + beta 6 antibody [10D5]
    See all Integrin alpha V + beta 6 primary antibodies
  • Description
    Mouse monoclonal [10D5] to Integrin alpha V + beta 6
  • Specificity
    ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins.
  • Tested applications
    Suitable for: IP, Flow Cyt, Blockingmore details
    Unsuitable for: IHC-P or WB
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Immunization of beta 6 knockout mice with wild type murine keratinocytes.

  • Positive control
    • H441 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab77906 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture.
Flow Cyt Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent.



ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
Blocking Use a concentration of 100 µg/ml. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP.
  • Application notes
    Is unsuitable for IHC-P or WB.
  • Target

    References

    This product has been referenced in:
    • de la Mare JA  et al. Extracellular Hsp90 and TGFß regulate adhesion, migration and anchorage independent growth in a paired colon cancer cell line model. BMC Cancer 17:202 (2017). Human . Read more (PubMed: 28302086) »
    • Bian Q  et al. Mechanosignaling activation of TGFß maintains intervertebral disc homeostasis. Bone Res 5:17008 (2017). Mouse . Read more (PubMed: 28392965) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    The exact target epitope was not characterized in-house. Please see the references listed above for more details.
    We do not have information on this product's reactivity with mouse as this antibody has, to our knowledge, not been tested on mouse sa...

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    Thank you for contacting us.

    This antibody (ab78438) recognizes the alpha V beta 5 heterodimer, not alpha V beta 6. We do however offer another product which does recognize alpha V beta 6. That product number is ab77906 and may be found at th...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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