Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free (ab77906)
Key features and details
- Mouse monoclonal [10D5] to Integrin alpha V + beta 6 - BSA and Azide free
- Suitable for: Flow Cyt, IP, Blocking
- Reacts with: Mouse, Rat, Human
- Isotype: IgG2a
Overview
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Product name
Anti-Integrin alpha V + beta 6 antibody [10D5] - BSA and Azide free
See all Integrin alpha V + beta 6 primary antibodies -
Description
Mouse monoclonal [10D5] to Integrin alpha V + beta 6 - BSA and Azide free -
Host species
Mouse -
Specificity
ab77906 recognises alpha V beta 6 and does not recognize alpha V or other alpha V integrins. -
Tested applications
Suitable for: Flow Cyt, IP, Blockingmore details
Unsuitable for: IHC-P or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Tissue, cells or virus corresponding to Mouse Integrin alpha V + beta 6. Wild type murine keratinocytes.
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Positive control
- H441 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Sterile filtered -
Clonality
Monoclonal -
Clone number
10D5 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use at an assay dependent concentration.
Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
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Blocking |
Use at an assay dependent concentration.
Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Flow cytometry should be done with buffers containing 1 mM Mg, since the epitope recognized by this antibody is divalent cation dependent. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. because this antibody is an IgG2a, protein G or rabbit anti mouse IgG is helpful for capture. |
Blocking
Use at an assay dependent concentration. Inhibition of alpha V beta 6 mediated cell adhesion and cell migration are achieved at a concentration of 100 µg/mL using beta 6 transfected cells tested against TGF beta LAP. |
Target
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Function
The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3685 Human
- Entrez Gene: 3694 Human
- Entrez Gene: 16410 Mouse
- Entrez Gene: 16420 Mouse
- Entrez Gene: 296456 Rat
- Entrez Gene: 311061 Rat
- Omim: 147558 Human
- Omim: 193210 Human
see all -
Alternative names
- CD51 antibody
- Integrin alpha-V light chain antibody
- integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51) antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (16)
ab77906 has been referenced in 16 publications.
- Wang X et al. Inhibition of Integrin αvβ6 Activation of TGF-β Attenuates Tendinopathy. Adv Sci (Weinh) 9:e2104469 (2022). PubMed: 35166070
- Harvey Y et al. An Improved αvβ6-Receptor-Expressing Suspension Cell Line for Foot-and-Mouth Disease Vaccine Production. Viruses 14:N/A (2022). PubMed: 35337028
- Gringhuis SI et al. Fungal sensing by dectin-1 directs the non-pathogenic polarization of TH17 cells through balanced type I IFN responses in human DCs. Nat Immunol 23:1735-1748 (2022). PubMed: 36456734
- Bieri M et al. The RGD-binding integrins αvβ6 and αvβ8 are receptors for mouse adenovirus-1 and -3 infection. PLoS Pathog 17:e1010083 (2021). PubMed: 34910784
- Hägglund S et al. Model of persistent foot-and-mouth disease virus infection in multilayered cells derived from bovine dorsal soft palate. Transbound Emerg Dis 67:133-148 (2020). PubMed: 31419374