Anti-Integrin beta 1 antibody (ab183666)
Key features and details
- Rabbit polyclonal to Integrin beta 1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
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- Research with confidence – consistent and reproducible results with every batch
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- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Integrin beta 1 antibody
See all Integrin beta 1 primary antibodies -
Description
Rabbit polyclonal to Integrin beta 1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig -
Immunogen
Synthetic peptide within Human Integrin beta 1 aa 738-798. The exact sequence is proprietary.
Database link: P05556 -
Positive control
- A431, HeLa, U87-MG, SK-N-SH, IMR32, SK-N-AS, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC12 and Rat-2 whole cell lysates/extracts. Human reast carcinoma and hepatoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183666 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/100 - 1/1000.
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WB | (1) |
1/5000 - 1/10000. Predicted molecular weight: 88 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
IHC-P
1/100 - 1/1000. |
WB
1/5000 - 1/10000. Predicted molecular weight: 88 kDa. |
Target
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Function
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling (PubMed:23125415, PubMed:24789099). ITGA4:ITGB1 and ITGA5:ITGB1 bind to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1 (PubMed:18635536, PubMed:25398877). ITGA5:ITGB1 acts as a receptor for fibrillin-1 (FBN1) and mediates R-G-D-dependent cell adhesion to FBN1 (PubMed:12807887, PubMed:17158881).
Isoform 5: Isoform 5 displaces isoform 1 in striated muscles.
(Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for human echoviruses 1 and 8 (PubMed:8411387). Acts as a receptor for cytomegalovirus/HHV-5 (PubMed:20660204). Acts as a receptor for Epstein-Barr virus/HHV-4 (PubMed:17945327). Integrin ITGA5:ITGB1 acts as a receptor for human parvovirus B19 (PubMed:12907437). Integrin ITGA2:ITGB1 acts as a receptor for human rotavirus (PubMed:12941907). Acts as a receptor for mammalian reovirus (PubMed:16501085). In case of HIV-1 infection, integrin ITGA5:ITGB1 binding to extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions (PubMed:10397733). -
Tissue specificity
Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma cells, hepatoma cells and astrocytoma cells. Isoform 3 and isoform 4 are expressed in muscle, kidney, liver, placenta, cervical epithelium, umbilical vein endothelial cells, fibroblast cells, embryonal kidney cells, platelets and several blood cell lines. Isoform 4, rather than isoform 3, is selectively expressed in peripheral T-cells. Isoform 3 is expressed in non-proliferating and differentiated prostate gland epithelial cells and in platelets, on the surface of erythroleukemia cells and in various hematopoietic cell lines. Isoform 5 is expressed specifically in striated muscle (skeletal and cardiac muscle). -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 VWFA domain. -
Post-translational
modificationsThe cysteine residues are involved in intrachain disulfide bonds. -
Cellular localization
Cell membrane, sarcolemma. Cell junction. In cardiac muscle, isoform 5 is found in costameres and intercalated disks and Cell membrane. Cell projection, invadopodium membrane. Cell projection, ruffle membrane. Recycling endosome. Melanosome. Cleavage furrow. Cell projection, lamellipodium. Cell projection, ruffle. Cell junction, focal adhesion. Cell surface. Isoform 2 does not localize to focal adhesions. Highly enriched in stage I melanosomes. Located on plasma membrane of neuroblastoma NMB7 cells. In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles. In early telophase, detected mainly on the matrix-facing side of the cells. By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow. In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells. Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen. Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner. Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner. Localized at plasma and ruffle membranes in a collagen-independent manner. - Information by UniProt
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Database links
- Entrez Gene: 3688 Human
- Entrez Gene: 16412 Mouse
- Entrez Gene: 397019 Pig
- Entrez Gene: 24511 Rat
- Omim: 135630 Human
- SwissProt: P05556 Human
- SwissProt: P09055 Mouse
- SwissProt: Q9GLP0 Pig
see all -
Alternative names
- beta1 integrin antibody
- CD_antigen=CD29 antibody
- CD29 antibody
see all
Images
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All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution
Lane 1 : A431 whole cell lysate/extract
Lane 2 : HeLa whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling Integrin beta 1 with ab183666 at 1/500 dilution.
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All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/10000 dilution
Lane 1 : U87-MG whole cell lysate/extract
Lane 2 : SK-N-SH whole cell lysate/extract
Lane 3 : IMR32 whole cell lysate/extract
Lane 4 : SK-N-AS whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
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ab183666 at 5μg immunoprecipitating Integrin beta 1 in HeLa whole cell extracts.
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All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : Neuro2A whole cell lysate/extract
Lane 2 : GL261 whole cell lysate/extract
Lane 3 : C8D30 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
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All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : NIH-3T3 whole cell lysate/extract
Lane 2 : BCL-1 whole cell lysate/extract
Lane 3 : Raw264.7 whole cell lysate/extract
Lane 4 : C2C12 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
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All lanes : Anti-Integrin beta 1 antibody (ab183666) at 1/5000 dilution
Lane 1 : PC-12 whole cell lysate/extract
Lane 2 : Rat-2 whole cell lysate/extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 88 kDa7.5 % SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded human Hepatoma tissue labeling Integrin beta 1 with ab183666 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab183666 has been referenced in 22 publications.
- Liu Y et al. SNARE protein USE1 is involved in the glycosylation and the expression of mumps virus fusion protein and important for viral propagation. PLoS Pathog 18:e1010949 (2022). PubMed: 36480520
- Kitano M et al. Rab11-mediated post-Golgi transport of the sialyltransferase ST3GAL4 suggests a new mechanism for regulating glycosylation. J Biol Chem 296:100354 (2021). PubMed: 33524390
- Li Y et al. Proteomic Analysis of Uterine Tissues During Peri-Implantation Period in Mice with Experimentally Induced Adenomyosis that Treated with anti-Ngf: Implications for Cell-Cell Adhesion and Metabolic Processes. Reprod Sci 28:207-217 (2021). PubMed: 32676925
- Quillen S et al. Astrocyte responses to experimental glaucoma in mouse optic nerve head. PLoS One 15:e0238104 (2020). PubMed: 32822415
- Zhu W et al. Knockout of EGFL6 by CRISPR/Cas9 Mediated Inhibition of Tumor Angiogenesis in Ovarian Cancer. Front Oncol 10:1451 (2020). PubMed: 32983976