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(C57BL/6 x DBA/2)F1 Mouse bone-marrow stromal cell clone BMS2.
Clone KMI6 has been shown to be an activating antibody for ß1 integrins, enhancing lymphocyte binding to fibronectin.
Our Abpromise guarantee covers the use of ab95623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 µg/ml. Use under non reducing condition. Predicted molecular weight: 88 kDa.|
|Flow Cyt||Use 0.125µg for 105-8 cells. Use a final volume of 100 µL.
ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
ICC/IF image of ab95623 stained MEF1 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95623, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab98420, a goat anti-rat DyLight® 488 (IgG; H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.