• Product name
    Anti-Integrin beta 1d antibody [2B1]
  • Description
    Mouse monoclonal [2B1] to Integrin beta 1d
  • Specificity
    Recognizes specifically the cytoplasmic domain of integrin subunit beta 1D present in cardiac and skeletal muscle.
  • Tested applications
    Suitable for: WB, IHC-Fr, ICCmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: C-QENPIYKSPINNFKNPNYGRKAGL conjugated to KLH.

  • Epitope
    Cytoplasmic domain, C-terminal. Isoform of the Integrin b1 subunit present in cardiac and skeletal muscle.
  • General notes

    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an alpha and a beta subunit in interconnecting the cytoskeleton and the extracellular matrix.
  • Clonality
  • Clone number
  • Myeloma
  • Isotype
  • Light chain type
  • Research areas


Our Abpromise guarantee covers the use of ab8991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000.
IHC-Fr 1/25 - 1/200.
ICC Use at an assay dependent concentration.


  • Relevance
    Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. The isoform beta-1D of Integrin 1, is expressed specifically in striated muscle (skeletal and cardiac muscle).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • CD29 antibody
    • FNRB antibody
    • Integrin beta 1 antibody
    • ITGB 1 antibody
    • ITGB1 antibody
    • MDF2 antibody
    • MSK12 antibody
    • VLAB antibody
    see all


  • Western blotting of beta1D integrin showing its muscle cell specific expression pattern.
  • Immunofluorescence staining of myocardium for beta1D integrin.


This product has been referenced in:
  • Barton ER  et al. Deletion of muscle GRP94 impairs both muscle and body growth by inhibiting local IGF production. FASEB J : (2012). ICC/IF ; Mouse . Read more (PubMed: 22649033) »
  • Barton ER Restoration of gamma-sarcoglycan localization and mechanical signal transduction are independent in murine skeletal muscle. J Biol Chem 285:17263-70 (2010). WB ; Mouse . Read more (PubMed: 20371873) »

See all 3 Publications for this product

Customer reviews and Q&As

Please forward the following publication to customer, there are many markers listed and we have antibodies against these. Customer might need to make decision based on their requirements.


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I would recommend to use an anti-integrin beta 1d such as http://www.abcam.com/ab8992 or http://www.abcam.com/ab8991.

Thank you for your enquiry. I am sorry it has taken a little time to obtain this protocol for you. Thank you for your patience. I hope this will be helpflu to you. Please note that this procedure would be a guideline only and may require some fur...

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We have just received the following information from the source of ab8991: "We would suggest to increase the protein concentration in the gel and to use more concentrated antibody for the blotting as this antibody is supplied as tissue culture supe...

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Thank you for your enquiry. For Western blotting, I would suggest starting at a dilution of 1:1000. For IHC, I would recommend titrating the antibody 1:20 to 1:200. For both applications, you will need to optimize based upon results. Please contact...

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We would expect this antibody to work on rat and mouse tissues, but cannot guarantee it. This epitope is conserved across species.


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