Overview

  • Product nameAnti-Integrin beta 3 antibody
    See all Integrin beta 3 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 3
  • Tested applicationsSuitable for: IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized non-phosphopeptide derived from human Integrin ß3 around the phosphorylation site of tyrosine 773.

  • Positive control
    • Human breast carcinoma tissue

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab47387 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/20000.

Target

  • FunctionIntegrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Tissue specificityIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Involvement in diseaseDefects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similaritiesBelongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    modifications
    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD 61 antibody
    • CD61 antibody
    • CD61 antigen antibody
    • GP3A antibody
    • GPIIIa antibody
    • GT antibody
    • HPA 1 antibody
    • HPA 4 antibody
    • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
    • Integrin beta chain beta 3 antibody
    • Integrin beta-3 antibody
    • ITB3_HUMAN antibody
    • ITG B3 antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • NAIT antibody
    • Platelet fibrinogen receptor beta subunit antibody
    • Platelet fibrinogen receptor, beta subunit antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • PTP antibody
    see all

Anti-Integrin beta 3 antibody images

  • Ab47387 at 1/50 dilution staining paraffin-embedded human breast carcinoma tissue. Right : peptide concentration 1ug/ml

References for Anti-Integrin beta 3 antibody (ab47387)

ab47387 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab47387.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"