Anti-Integrin beta 3 antibody [PM6/13] (Phycoerythrin) (ab33272)

Overview

  • Product nameAnti-Integrin beta 3 antibody [PM6/13] (Phycoerythrin)
    See all Integrin beta 3 primary antibodies
  • Description
    Mouse monoclonal [PM6/13] to Integrin beta 3 (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human, Baboon
  • Immunogen

    Platelet plasma membrane

  • Positive control
    • human peripheral blood platelets

Properties

Applications

Our Abpromise guarantee covers the use of ab33272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
ELISA
  • Application notesELISA: Use at an assay dependent dilution (PMID 18516040).
    Flow Cyt: Use 10µl for 106 cells.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionIntegrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
    • Tissue specificityIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
    • Involvement in diseaseDefects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
    • Sequence similaritiesBelongs to the integrin beta chain family.
      Contains 1 VWFA domain.
    • Post-translational
      modifications
      Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • BDPLT16 antibody
      • BDPLT2 antibody
      • CD 61 antibody
      • CD61 antibody
      • CD61 antigen antibody
      • GP3A antibody
      • GPIIIa antibody
      • GT antibody
      • HPA 1 antibody
      • HPA 4 antibody
      • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
      • Integrin beta chain beta 3 antibody
      • Integrin beta-3 antibody
      • ITB3_HUMAN antibody
      • ITG B3 antibody
      • ITGB 3 antibody
      • ITGB3 antibody
      • NAIT antibody
      • Platelet fibrinogen receptor beta subunit antibody
      • Platelet fibrinogen receptor, beta subunit antibody
      • Platelet glycoprotein IIIa antibody
      • Platelet glycoprotein IIIa precursor antibody
      • Platelet membrane glycoprotein IIIa antibody
      • PTP antibody
      see all

    Anti-Integrin beta 3 antibody [PM6/13] (Phycoerythrin) images

    • Neat ab33272 staining human peripheral blood platelets.

    References for Anti-Integrin beta 3 antibody [PM6/13] (Phycoerythrin) (ab33272)

    This product has been referenced in:
    • Gerold G  et al. A Toll-like receptor 2-integrin beta3 complex senses bacterial lipopeptides via vitronectin. Nat Immunol 9:761-8 (2008). ELISA, Flow Cyt ; Human . Read more (PubMed: 18516040) »

    See 1 Publication for this product

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