Anti-Integrin beta 3 (phospho Y773) antibody (ab5190)


  • Product nameAnti-Integrin beta 3 (phospho Y773) antibody
    See all Integrin beta 3 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 3 (phospho Y773)
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus laevis
  • Immunogen

    The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of human Integrin beta 3 receptor that contains tyrosine 773 (based on Swiss Protein database, accession number P05106). The sequence is conserved in human, mouse and rat.

  • Positive control
    • K562 cells transfected with human Integrin alpha and wild-type (WT) or mutant (Y773F) human Integrin beta 3, and analysed by immunoprecipitation with 1A2 (human Integrin beta 3) mAb.



Our Abpromise guarantee covers the use of ab5190 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 1 µg/ml. Predicted molecular weight: 130 kDa.


  • FunctionIntegrin alpha-V/beta-3 is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. Integrins alpha-IIb/beta-3 and alpha-V/beta-3 recognize the sequence R-G-D in a wide array of ligands. Integrin alpha-IIb/beta-3 recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions.
  • Tissue specificityIsoform beta-3A and isoform beta-3C are widely expressed. Isoform beta-3A is specifically expressed in osteoblast cells; isoform beta-3C is specifically expressed in prostate and testis.
  • Involvement in diseaseDefects in ITGB3 are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors.
  • Sequence similaritiesBelongs to the integrin beta chain family.
    Contains 1 VWFA domain.
  • Post-translational
    Phosphorylated on tyrosine residues in response to thrombin-induced platelet aggregation. Probably involved in outside-in signaling. A peptide (AA 740-762) is capable of binding GRB2 only when both Tyr-773 and Tyr-785 are phosphorylated. Phosphorylation of Thr-779 inhibits SHC binding.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BDPLT16 antibody
    • BDPLT2 antibody
    • CD 61 antibody
    • CD61 antibody
    • CD61 antigen antibody
    • GP3A antibody
    • GPIIIa antibody
    • GT antibody
    • HPA 1 antibody
    • HPA 4 antibody
    • Integrin beta 3 (platelet glycoprotein IIIa antigen CD61) antibody
    • Integrin beta chain beta 3 antibody
    • Integrin beta-3 antibody
    • ITB3_HUMAN antibody
    • ITG B3 antibody
    • ITGB 3 antibody
    • ITGB3 antibody
    • NAIT antibody
    • Platelet fibrinogen receptor beta subunit antibody
    • Platelet fibrinogen receptor, beta subunit antibody
    • Platelet glycoprotein IIIa antibody
    • Platelet glycoprotein IIIa precursor antibody
    • Platelet membrane glycoprotein IIIa antibody
    • PTP antibody
    see all

Anti-Integrin beta 3 (phospho Y773) antibody images

  • Predicted band size : 130 kDa

    Western Blot: K562 cells transfected with human Integrin áv and wild-type (WT) or mutant (Y773F) human Integrin beta 3 were analyzed by immunoprecipitating the Integrin beta 3 protein with 1A2 (human Integrin beta 3) mAb, and resolved by SDS-PAGE on a 10% Tris-glycine gel. The proteins then were transferred to PVDF and, after blocking with a 5% BSA-TBST buffer overnight at 4oC, incubated with 0.50 µg/mL Integrin beta 3 [pY773] antibody with samples corresponding to: non-phosphorylated Integrin beta 3 (1), phosphorylated WT Integrin beta 3 (2), or phosphorylated Y773F mutant Integrin beta 3 (3). After washing, membranes were incubated with goat F(ab’)2 antirabbit IgG alkaline phosphatase and bands were detected using the Tropix WesternStar method. The data show detection of Integrin beta 3 receptor phosphorylation with the wild-type but not the mutant recombinant protein, thereby demonstrating the specificity of the Integrin 3 [pY773] antibody.

References for Anti-Integrin beta 3 (phospho Y773) antibody (ab5190)

ab5190 has not yet been referenced specifically in any publications.

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Application Western blot
Sample Human Cell lysate - other (HUVEC/ membrane fractions)
Loading amount 10 µg
Specification HUVEC/ membrane fractions
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

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Submitted Feb 02 2007