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Full length native protein (purified) (Mouse) - Tumour associated antigen TSP 180 - immunoaffinity purified from a transplantable BALB/c mouse lung cell carcinoma.
Our Abpromise guarantee covers the use of ab25254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 20094062|
|IHC-Fr||Use a concentration of 1 - 5 µg/ml.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.|
Immunohistochemistry (Frozen sections) analysis of BALB/c mouse frozen intestine. Right: Integrin beta 4 labeled with ab25254 followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Left: Isotype Control, Rat IgG2a-UNLB followed by Goat Anti-Rat IgG(H+L), Mouse ads-BIOT, and Streptavidin-FITC. Blue - DAPI
ab25254 staining mouse small intestine tissue sections by IHC-Fr. Sections were PFA fixed and blocked in 3% BSA for 1 hour at 37°C prior to incubatition with the primary antibody, diluted 1/500, for 12 hours at 4°C. A Cy3® conjugated goat anti-rat antibody, diluted 1/200, was used as the secondary.
ab25254 staining Intergrin beta 4 in mouse colon cells by Flow Cytometry. The sample was incubated with the primary antibody (1/100) for 30 minutes at 20°C. An undiluted Alexa Fluor® 647-conjugated goat anti-rat IgG polyclonal was used as the secondary antibody. Gating Strategy: Exclude small cells/debris.