Anti-Integrin beta 4 antibody [346-11A] (Biotin) (ab54414)

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Overview

  • Product name
    Anti-Integrin beta 4 antibody [346-11A] (Biotin)
    See all Integrin beta 4 primary antibodies
  • Description
    Rat monoclonal [346-11A] to Integrin beta 4 (Biotin)
  • Host species
    Rat
  • Conjugation
    Biotin
  • Specificity
    This antibody is specific to mouse CD104
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tumour Surface Protein-180 purified from Balb/c murine carcinoma.

  • Positive control
    • Mouse thymocytes
  • General notes
    Shelf Life - 18 months, product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purified from tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    346-11A
  • Myeloma
    P3-x63-Ag8
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab54414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use neat or to a 1/5 dilution. Use 10µl of the suggested working dilution to label 106 cells in 100µl

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Function
      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • Tissue specificity
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • Involvement in disease
      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • Domain
      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
      • Alternative names
        • CD 104 antibody
        • CD104 antibody
        • CD104 antigen antibody
        • gp150 antibody
        • Integrin beta 4 subunit antibody
        • Integrin beta-4 antibody
        • ITB4_HUMAN antibody
        • ITG B4 antibody
        • ITGB 4 antibody
        • Itgb4 antibody
        see all

      Images

      • Flow Cytometry - Integrin beta 4 antibody [346-11A] (Biotin) (ab54414)
        Flow Cytometry - Integrin beta 4 antibody [346-11A] (Biotin) (ab54414)
        ab54414, used neat, staining mouse thymocytes using Flow Cytometry

      References

      ab54414 has not yet been referenced specifically in any publications.

      Publishing research using ab54414? Please let us know so that we can cite the reference in this datasheet.

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