Anti-Integrin beta 4 antibody [346-11A] (Biotin) (ab54414)

Overview

• Product name
  Anti-Integrin beta 4 antibody [346-11A] (Biotin)
  See all Integrin beta 4 primary antibodies
• Description
  Rat monoclonal [346-11A] to Integrin beta 4 (Biotin)
• Conjugation
  Biotin
• Specificity
  This antibody is specific to mouse CD104
• Tested applications
  Suitable for: Flow Cytmore details
• Species reactivity
  Reacts with: Mouse
  
• Immunogen

  Tumour Surface Protein-180 purified from Balb/c murine carcinoma.

• Positive control
  • Mouse thymocytes
• General notes
  Shelf Life - 18 months, product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

Properties

• Form
  Liquid
• Storage instructions
  Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
• Storage buffer
  Preservative: 0.09% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• Purity
  Protein G purified
• Purification notes
  Purified from tissue culture supernatant
• Clonality
  Monoclonal
• Clone number
  346-11A
• Myeloma
  P3-x63-Ag8
• Isotype
  IgG2a
• Research areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cell Adhesion
  • Integrins
  • Beta

Associated products

• Compatible Secondaries
  • Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157)
  • Goat Anti-Rat IgG H&L (HRP) (ab205720)

Applications

Target

• Function
  Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
• Tissue specificity
  Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
• Involvement in disease
  Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
  Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
• Sequence similarities
  Belongs to the integrin beta chain family.
  Contains 1 Calx-beta domain.
  Contains 4 fibronectin type-III domains.
  Contains 1 PSI domain.
  Contains 1 VWFA domain.
• Domain
  The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
• Cellular localization
  Membrane.

• Target information above from: UniProt accession P16144 The UniProt Consortium
  The Universal Protein Resource (UniProt) in 2010
  Nucleic Acids Res. 38:D142-D148 (2010) .

  Information by UniProt
• Database links
  • Entrez Gene: 192897 Mouse
  • SwissProt: A2A863 Mouse
  • Unigene: 213873 Mouse
• Alternative names
  • CD 104 antibody
  • CD104 antibody
  • CD104 antigen antibody

  • gp150 antibody
  • Integrin beta 4 subunit antibody
  • Integrin beta-4 antibody
  • ITB4_HUMAN antibody
  • ITG B4 antibody
  • ITGB 4 antibody
  • Itgb4 antibody

  see all

Protocols

• Flow cytometry protocols