Anti-Integrin beta 4 antibody [439-9B] (ab110167)

Overview

  • Product nameAnti-Integrin beta 4 antibody [439-9B]
    See all Integrin beta 4 primary antibodies
  • Description
    Rat monoclonal [439-9B] to Integrin beta 4
  • SpecificityRecognizes human Integrin beta-4, a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified.
  • Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Other Immunogen Type corresponding to Human Integrin beta 4.
    Database link: P16144

  • Positive control
    • Human Pancreas and Human Placenta tissues, A549 cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • PurityAffinity purified
  • ClonalityMonoclonal
  • Clone number439-9B
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab110167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.
IHC-P Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 1µg for 105-8 cells. in 100 µl



ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similaritiesBelongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [439-9B] images

  • ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Pancreas(ab29816). Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

  • ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Placenta. Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.

  • Staining of the A549 cell line with 0.5 ug of Rat IgG2b Isotype Control (ab98984) (blue histogram) or 0.5 ug of ab110167 (purple histogram) followed by Biotin Anti-Rat IgG and Sav-PE. Total viable cells were used for analysis.

References for Anti-Integrin beta 4 antibody [439-9B] (ab110167)

ab110167 has not yet been referenced specifically in any publications.

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