Anti-Integrin beta 4 antibody [439-9B] (ab110167)
Key features and details
- Rat monoclonal [439-9B] to Integrin beta 4
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Related conjugates and formulations
Overview
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Product name
Anti-Integrin beta 4 antibody [439-9B]
See all Integrin beta 4 primary antibodies -
Description
Rat monoclonal [439-9B] to Integrin beta 4 -
Host species
Rat -
Specificity
Recognizes human Integrin beta-4, a 202 kDa subunit that associates with integrin alpha 6 (CD49f), thereby forming the laminin receptor. Multiple splice variants have been identified. It is used as a surface staining antibody.
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Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
The details of the immunogen for this antibody are not available.
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Positive control
- Human Pancreas and Human Placenta tissues, A549 cell line
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
439-9B -
Isotype
IgG2b -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab110167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt |
Use 1µg for 105-8 cells.
in 100 µl
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Notes |
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IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
Use 1µg for 105-8 cells. in 100 µl
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells. -
Tissue specificity
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach. -
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities. -
Sequence similarities
Belongs to the integrin beta chain family.
Contains 1 Calx-beta domain.
Contains 4 fibronectin type-III domains.
Contains 1 PSI domain.
Contains 1 VWFA domain. -
Domain
The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3691 Human
- Omim: 147557 Human
- SwissProt: P16144 Human
- Unigene: 632226 Human
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Alternative names
- CD 104 antibody
- CD104 antibody
- CD104 antigen antibody
see all
Images
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ab110167 at 20 µg/ml staining integrin beta 4 in Formalin-Fixed, Paraffin-Embedded Human Placenta. Sections were incubated with a biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab110167 has been referenced in 10 publications.
- Walker MR et al. O-linked α2,3 sialylation defines stem cell populations in breast cancer. Sci Adv 8:eabj9513 (2022). PubMed: 34995107
- Choi SH et al. KRAS Mutants Upregulate Integrin β4 to Promote Invasion and Metastasis in Colorectal Cancer. Mol Cancer Res 20:1305-1319 (2022). PubMed: 35394541
- Riivari S et al. Epithelial cell attachment and adhesion protein expression on novel in sol TiO2 coated zirconia and titanium alloy surfaces. J Biomed Mater Res B Appl Biomater 110:2533-2541 (2022). PubMed: 35730701
- Wang M et al. Centrosome loss results in an unstable genome and malignant prostate tumors. Oncogene 39:399-413 (2020). PubMed: 31477840
- Urciuolo A et al. Intravital three-dimensional bioprinting. Nat Biomed Eng 4:901-915 (2020). PubMed: 32572195
- Wang M et al. Integrin a6ß4E variant is associated with actin and CD9 structures and modifies the biophysical properties of cell-cell and cell-extracellular matrix interactions. Mol Biol Cell 30:838-850 (2019). PubMed: 30865564
- Brown CW et al. The a6ß4 integrin promotes resistance to ferroptosis. J Cell Biol 216:4287-4297 (2017). PubMed: 28972104
- Muranen T et al. Starved epithelial cells uptake extracellular matrix for survival. Nat Commun 8:13989 (2017). Mouse, Human . PubMed: 28071763
- An XZ et al. Netrin-1 suppresses the MEK/ERK pathway and ITGB4 in pancreatic cancer. Oncotarget 7:24719-33 (2016). PubMed: 27034160
- van Essen TH et al. Biocompatibility of a fish scale-derived artificial cornea: Cytotoxicity, cellular adhesion and phenotype, and in vivo immunogenicity. Biomaterials 81:36-45 (2016). PubMed: 26717247