Anti-Integrin beta 4 antibody [439-9B] (Biotin) (ab95584)

Overview

  • Product name
    Anti-Integrin beta 4 antibody [439-9B] (Biotin)
    See all Integrin beta 4 primary antibodies
  • Description
    Rat monoclonal [439-9B] to Integrin beta 4 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Cow, Human
  • Immunogen

    CD104 (Integrin beta 4)

  • Positive control
    • EDTA treated A549 cell line
  • General notes


    ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein G purified
  • Primary antibody notes
    ab95584 has been reported to have no effect on adhesion of Human colon adenocarcinoma cells
  • Clonality
    Monoclonal
  • Clone number
    439-9B
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 0.25µg for 105-8 cells. ab18542-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [439-9B] (Biotin) images

  • Staining of the A549 cell line with 0.125 µg of Rat IgG2b ? Isotype Control Biotin (open histogram) or 0.125 µg of ab95584 (filled histogram) followed by Streptavidin PE. Total viable cells were used for analysis.

References for Anti-Integrin beta 4 antibody [439-9B] (Biotin) (ab95584)

This product has been referenced in:
  • Simon-Assmann P  et al. Adhesive properties and integrin expression profiles of two colonic cancer populations differing by their spreading on laminin. J Cell Sci 107 ( Pt 3):577-87 (1994). Read more (PubMed: 8006072) »
  • Falcioni R  et al. Monoclonal antibody to human carcinoma-associated protein complex: quantitation in normal and tumor tissue. Cancer Res 48:816-21 (1988). Read more (PubMed: 2448027) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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