Anti-Integrin beta 4 antibody [450-9D] (FITC) (ab22486)

Overview

  • Product nameAnti-Integrin beta 4 antibody [450-9D] (FITC)
    See all Integrin beta 4 primary antibodies
  • Description
    Mouse monoclonal [450-9D] to Integrin beta 4 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab22486 recognises the integrin beta 4 expressed on epithelial cells, Schwann cells and various tumour cell lines.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) (Human): Purified a6b4 integrin from A431 cells

  • Epitopethe epitope is in the extracellular region of Integrin beta 4
  • Positive control
    • Human tonsil

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab22486 has been purified by affinity chromatography on Protein G from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone number450-9D
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt 1/10. Use 10ul of the suggested working dilution to label 106 cells in 100ul.



ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similaritiesBelongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • DomainThe fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 antibody [450-9D] (FITC) images

  • Immunohistochemistry (Frozen sections) analysis of human tonsil tissue section labeling Integrin beta 4 with ab22486. 

References for Anti-Integrin beta 4 antibody [450-9D] (FITC) (ab22486)

ab22486 has not yet been referenced specifically in any publications.

Product Wall

Further to previous correspondence I have been in touch with the source of this antibody. I am concerned that this antibody has in fact NOT been tested and shown to work by western blotting. It is FITC labeled and therefore my concern was that the fluo...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I appreciate the controls that you have in place and that you are detecting Integrin beta 4 using an alternatively sourced antibody. Please ca...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"