Anti-Integrin beta 4 antibody [4509D] - Azide free (ab186719)

Overview

  • Product name
    Anti-Integrin beta 4 antibody [4509D] - Azide free
    See all Integrin beta 4 primary antibodies
  • Description
    Mouse monoclonal [4509D] to Integrin beta 4 - Azide free
  • Host species
    Mouse
  • Specificity
    ab186719 recognises an extracellular epitope on the Integrin beta 4 molecule.
  • Tested applications
    Suitable for: Flow Cyt, ICC/IF, IHC-Fr, IP, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human, Ferret, Mink
    Predicted to work with: Mouse
  • Immunogen

    Full length native protein (purified) corresponding to Human Integrin beta 4. Purified from A431 cells.
    Database link: P16144

  • Positive control
    • Human tonsil tissue.
  • General notes

    Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    ab186719 was prepared by affinity chromatography on Protein G from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    4509D
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/500 - 1/1000.

The use of acetone fixation for frozen sections is recommended.

IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 202 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • Tissue specificity
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • Involvement in disease
      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • Domain
      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Images

    • Immunohistochemical analysis of frozen Human tonsil tissue labeling Integrin beta 4 with ab186719 at 1/500 dilution.

    References

    ab186719 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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