Overview

  • Product name
    Anti-Integrin beta 4 antibody [ASC-8]
    See all Integrin beta 4 primary antibodies
  • Description
    Mouse monoclonal [ASC-8] to Integrin beta 4
  • Tested applications
    Suitable for: IP, IHC-Fr, Flow Cyt, Inhibition Assaymore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human squamous cell carcinoma cells (SCC9)

  • Positive control
    • IHC-Fr: Human epithelial tissues (including tongue, foreskin and ovary). Flow Cyt: Human SCC9, SKOV-3, HUVEC cell lines. Does not stain monocytes, neutrophils, lymphocytes, T cells, B cells or platelets.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 0.25M Sodium chloride, 0.02M PBS, pH 7.6
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    ASC-8
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution. Precipitates PAGE bands of 66, 76, 120, 135 and 200kDa (reduced) or 66, 110, 125, 130 and 190kDa (non-reduced) from surface-biotinylated SCC9 cells.
IHC-Fr Use at an assay dependent dilution.
Flow Cyt Use 1µg for 106 cells.
Inhibition Assay Use at an assay dependent dilution. Inhibits SCC9 cell adhesion to human and EHS laminin and laminin GD-2 peptide, but not to type IV collagen or fibronectin. Does not inhibit SKOV-3 cell adhesion to laminin, fibronectin or type IV collagen.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • Tissue specificity
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • Involvement in disease
      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • Domain
      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all

    Images

    • Overlay histogram showing A431 cells stained with ab77801 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77801, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References

    This product has been referenced in:
    • Chen Q  et al. Down-regulation of Gli transcription factor leads to the inhibition of migration and invasion of ovarian cancer cells via integrin ß4-mediated FAK signaling. PLoS One 9:e88386 (2014). WB ; Human . Read more (PubMed: 24533083) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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