Validated using a knockout cell line
Recombinant
RabMAb

Anti-Integrin beta 4 antibody [EPR17517] (ab182120)

Overview

  • Product name
    Anti-Integrin beta 4 antibody [EPR17517]
    See all Integrin beta 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR17517] to Integrin beta 4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Mouse Integrin beta 4 aa 1600 to the C-terminus. The exact sequence is proprietary.
    Database link: A2A863

  • Positive control
    • WB: Mouse spleen, kidney, pancreas, ovary and skin lysates; Human skin and pancreas lysates; BxPC-3 and SW480 whole cell lysates. IHC-P: Human colon, Human cervical carcinoma and mouse pancreas tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR17517
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab182120 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 210 kDa (predicted molecular weight: 202 kDa).

Target

  • Function
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Images



  • Predicted band size : 202 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (40 µg)              
    Lane 2: ITGB4 knockout  HAP1 whole cell lysate (40 µg)

    Lanes 1 - 2: Merged signal (red and green). Green - ab182120 observed at 240 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab182120 was shown to specifically recognize ITGB4 in wild-type HAP1 cells alond with additional cross-reactive bands. No band was observed when ITGB4 knockout samples were used. Wild-type and ITGB4 knockout samples were subjected to SDS-PAGE.  Ab182120 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/30,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human cervical carcinoma is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cell junction staining on mouse pancreatic acinus is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab182120 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Membrane and cytoplasm staining on Human colon epithelium is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

  • All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : Mouse kidney lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution

    Lane 1 : Mouse pancreas lysate
    Lane 2 : Mouse ovary lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Mouse skin lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/20000 dilution + Human skin lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + Human pancreas lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/1000 dilution + BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Integrin beta 4 antibody [EPR17517] (ab182120) at 1/5000 dilution + SW480 (Human colon adenocarcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 202 kDa
    Observed band size : 210 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

References

ab182120 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formalin
Username

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Verified customer

Submitted Jun 23 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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