Anti-Integrin beta 4 antibody [EPR8558(2)] (ab168386)


  • Product name
    Anti-Integrin beta 4 antibody [EPR8558(2)]
    See all Integrin beta 4 primary antibodies
  • Description
    Rabbit monoclonal [EPR8558(2)] to Integrin beta 4
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human Integrin beta 4 (UniProt: P16144).

  • Positive control
    • BxPC3, SW480, A431 and HT29 cell lysates; Human colon and placenta tissues.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
  • Clone number
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab168386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 110-210 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
    • Tissue specificity
      Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
    • Involvement in disease
      Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
      Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
    • Sequence similarities
      Belongs to the integrin beta chain family.
      Contains 1 Calx-beta domain.
      Contains 4 fibronectin type-III domains.
      Contains 1 PSI domain.
      Contains 1 VWFA domain.
    • Domain
      The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 104 antibody
      • CD104 antibody
      • CD104 antigen antibody
      • gp150 antibody
      • Integrin beta 4 subunit antibody
      • Integrin beta-4 antibody
      • ITB4_HUMAN antibody
      • ITG B4 antibody
      • ITGB 4 antibody
      • Itgb4 antibody
      see all


    • ab168386 showing +ve staining in Human normal spleen.
    • ab168386 showing +ve staining in Human skin.
    • All lanes : Anti-Integrin beta 4 antibody [EPR8558(2)] (ab168386) at 1/1000 dilution

      Lane 1 : BxPC3 cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HT29 cell lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 110-210 kDa

    • Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Integrin beta 4 with ab168386 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling Integrin beta 4 with ab168386 at 1/50 dilution.


    ab168386 has not yet been referenced specifically in any publications.

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