The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Use at an assay dependent concentration. PubMed: 21042878
FunctionIntegrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Tissue specificityIntegrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in diseaseDefects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa. Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Immunocytochemistry/ Immunofluorescence - Anti-Integrin beta 4 antibody [M126] (ab29042)Image from Hiroyasu S et al., Am J Pathol., 182, 828-40. Fig 1.; doi: 10.1016/j.ajpath.2012.11.029.
Immunofluorescence analysis of rat 804G cells, staining Integrin beta 4 with ab29042.
Cells were fixed in 4% formaldehyde in PBS for 8 minutes, washed thoroughly in PBS, and permeabilized in 0.1% Triton X-100 in PBS for 10 minutes. Samples were incubated with primary antibody at 37°C for 1 hour. The cells on coverslips were washed with PBS, and AlexaFluor®597-conjugated secondary antibodies were applied for 1 hour at 37°C.
Western blot - Integrin beta 4 antibody [M126] (ab29042)
All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)
Lane 1 : A431 (25 µg/lane) serum starved overnight Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min
Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
References for Anti-Integrin beta 4 antibody [M126] (ab29042)
This product has been referenced in:
Montagner A et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med6:80-98 (2014).
Read more (PubMed: 24203162) »
Xiang Y et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One9:e95533 (2014).
Read more (PubMed: 24740264) »