Overview

  • Product name
    Anti-Integrin beta 4 antibody [M126]
    See all Integrin beta 4 primary antibodies
  • Description
    Mouse monoclonal [M126] to Integrin beta 4
  • Tested applications
    Suitable for: ELISA, WB, ICC, Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Dog, Human
  • Immunogen

    recombinant protein containing amino acid residues in the cytoplasmic region of human integrin beta 4.

  • Positive control
    • A431 cell lysate. 804G cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    protein A chromatography.
  • Clonality
    Monoclonal
  • Clone number
    M126
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab29042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB 1/1000. Predicted molecular weight: 202 kDa. In 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature.
ICC 1/250.
Flow Cyt 1/100. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 21042878

Target

  • Function
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Images

  • Immunofluorescence analysis of rat 804G cells, staining Integrin beta 4 with ab29042.

    Cells were fixed in 4% formaldehyde in PBS for 8 minutes, washed thoroughly in PBS, and permeabilized in 0.1% Triton X-100 in PBS for 10 minutes. Samples were incubated with primary antibody at 37°C for 1 hour. The cells on coverslips were washed with PBS, and AlexaFluor®597-conjugated secondary antibodies were applied for 1 hour at 37°C.

  • All lanes : Anti-Integrin beta 4 antibody [M126] (ab29042)

    Lane 1 : A431 (25 µg/lane) serum starved overnight
    Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min


    Predicted band size : 202 kDa
  • Overlay histogram showing A431 cells stained with ab29042 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum (ab7481) / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab29042, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG; H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

References

This product has been referenced in:
  • Montagner A  et al. Src is activated by the nuclear receptor peroxisome proliferator-activated receptor ß/d in ultraviolet radiation-induced skin cancer. EMBO Mol Med 6:80-98 (2014). IF . Read more (PubMed: 24203162) »
  • Xiang Y  et al. Analysis on the relevance of asthma susceptibility with the alteration of integrin ß 4 expression. PLoS One 9:e95533 (2014). WB ; Human . Read more (PubMed: 24740264) »

See all 8 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (Rabbit cornea. Limbal area.)
Permeabilization
No
Specification
Rabbit cornea. Limbal area.
Blocking step
5% BSA + 10% FBS in PBT (PBS + 0,1% triton) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone
Username

Dr. Noelia Andollo

Verified customer

Submitted Jul 20 2015

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Glioma cells)
Specification
Glioma cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 27 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
100000 cells
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted May 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (EpiSCs)
Specification
EpiSCs
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton 0.1%
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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