Anti-eIF-6 antibody (ab109593)
Key features and details
- Rabbit polyclonal to eIF-6
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-eIF-6 antibody
See all eIF-6 primary antibodies -
Description
Rabbit polyclonal to eIF-6 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Sheep, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Catfish, Rainbow trout, Orangutan -
Immunogen
Synthetic peptide corresponding to Human eIF-6 aa 150-200.
-
Positive control
- HeLa or NIH3T3 whole cell lysate; IHC: mouse renal cell carcinoma (renca positive), human ovarian carcinoma
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/10000. Predicted molecular weight: 27 kDa.
|
|
IP |
Use at 2-5 µg/mg of lysate.
|
|
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
Notes |
---|
WB
1/2000 - 1/10000. Predicted molecular weight: 27 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
-
Function
Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity. -
Tissue specificity
Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level). -
Sequence similarities
Belongs to the eIF-6 family. -
Post-translational
modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus. - Information by UniProt
-
Database links
- Entrez Gene: 286811 Cow
- Entrez Gene: 3692 Human
- Entrez Gene: 16418 Mouse
- Entrez Gene: 305506 Rat
- Entrez Gene: 100302338 Sheep
- Omim: 602912 Human
- SwissProt: Q9TU47 Cow
- SwissProt: P56537 Human
see all -
Alternative names
- b(2)gcn antibody
- B(2)GCN homolog antibody
- B4 integrin interactor antibody
see all
Images
-
Immunohistochemistry analysis of formalin-fixed paraformaldehyde-fixed mouse renca positive renal cell carcinoma tissue staining eIF-6 with ab109593 with a 1/1000 dilution. Counterstained with hematoxylin
-
Immunohistochemistry analysis of formalin-fixed paraformaldehyde-fixed human ovarian carcinoma tissue staining eIF-6 with ab109593 with a 1/1000 dilution. Counterstained with hematoxylin
-
All lanes : Anti-eIF-6 antibody (ab109593) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 3 minutes -
Detection of eIF-6 by Western Blot of Immunprecipitate.
Lane 1: ab109593 at 1µg/ml staining eIF-6 in HeLa whole cell lysate immunoprecipitated using ab109593 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab109593 has not yet been referenced specifically in any publications.