FunctionBinds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
Tissue specificityExpressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
Sequence similaritiesBelongs to the eIF-6 family.
Post-translational modificationsPhosphorylation at Ser-174 and Ser-175 promotes nuclear export.
Cellular localizationCytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
Anti-Integrin beta 4 binding protein antibody images
Western blot - integrin beta 4 binding protein antibody (ab109593)
All lanes : Anti-Integrin beta 4 binding protein antibody (ab109593) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : NIH3T3 whole cell lysate at 50 µg
developed using the ECL technique
Predicted band size : 27 kDa
Exposure time : 3 minutes
Immunoprecipitation - integrin beta 4 binding protein antibody (ab109593)
Detection of integrin beta 4 binding protein by Western Blot of Immunprecipitate.
Lane 1: ab109593 at 1µg/ml staining integrin beta 4 binding protein in HeLa whole cell lysate immunoprecipitated using ab109593 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 10 seconds.
References for Anti-Integrin beta 4 binding protein antibody (ab109593)
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