Recombinant
RabMAb

Anti-Integrin beta 4 binding protein antibody [EPR6512(B)] (ab124839)

Overview

  • Product name
    Anti-Integrin beta 4 binding protein antibody [EPR6512(B)]
    See all Integrin beta 4 binding protein primary antibodies
  • Description
    Rabbit monoclonal [EPR6512(B)] to Integrin beta 4 binding protein
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human integrin beta 4 binding protein protein (P56537).

  • Positive control
    • HeLa, RAW 264.7, K562, 293T, and HepG2 cell lysates and Human colonic adenocarcinoma tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab124839 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
Flow Cyt 1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit to form the 80S initiation complex in the cytoplasm. May behave as a stimulatory translation initiation factor downstream insulin/growth factors. Is also involved in ribosome biogenesis. Associates with pre-60S subunits in the nucleus and is involved in its nuclear export. Cytoplasmic release of TIF6 from 60S subunits and nuclear relocalization is promoted by a RACK1 (GNB2L1)-dependent protein kinase C activity.
    • Tissue specificity
      Expressed at very high levels in colon carcinoma with lower levels in normal colon and ileum and lowest levels in kidney and muscle (at protein level).
    • Sequence similarities
      Belongs to the eIF-6 family.
    • Post-translational
      modifications
      Phosphorylation at Ser-174 and Ser-175 promotes nuclear export.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Shuttles between cytoplasm and nucleus/nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • b(2)gcn antibody
      • B(2)GCN homolog antibody
      • B4 integrin interactor antibody
      • Binding protein of beta-4 integrin antibody
      • CAB antibody
      • eIF-6 antibody
      • EIF3A antibody
      • EIF6 antibody
      • Eukaryotic translation initiation factor 3A antibody
      • Eukaryotic translation initiation factor 6 antibody
      • IF6_HUMAN antibody
      • ITGB4BP antibody
      • OK/SW-cl.27 antibody
      • p27 beta 4 integrin binding protein antibody
      • p27(BBP) antibody
      • p27BBP antibody
      • RP4-614O4.1 antibody
      see all

    Images

    • All lanes : Anti-Integrin beta 4 binding protein antibody [EPR6512(B)] (ab124839) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : RAW 264.7 cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 27 kDa
    • ab124839, at 1/100 to 1/250, staining integrin beta 4 binding protein in Human colonic adenocarcinoma by immunohistochemistry [Paraffin Embedded Tissues (IHC-P)].

    References

    ab124839 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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