The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 Block the membrane in 5% BSA (fraction V). Predicted molecular weight: 202 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
Involvement in disease
Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa. Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
Western blot - Integrin beta 4 (phospho Y1494) antibody (ab29043)
All lanes : Anti-Integrin beta 4 (phospho Y1494) antibody (ab29043)
Lane 1 : A431 (25 µg/lane) serum starved overnight Lane 2 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with absence of peptide Lane 3 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1494) peptide Lane 4 : A431 (25 µg/lane) serum treated with pervanadate (1 mM) for 30 min with presence of phospho-integrin-beta4 (Tyr-1526) peptide
Liu SY et al. A small molecule induces integrin ß4 nuclear translocation and apoptosis selectively in cancer cells with high expression of integrin ß4. Oncotarget7:16282-96 (2016).
Read more (PubMed: 26918348) »