Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)

Overview

  • Product name
    Anti-Integrin beta 4 (phospho Y1526) antibody
    See all Integrin beta 4 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 4 (phospho Y1526)
  • Specificity
    The immunogen sequence used is found in all three integrin beta4 isoforms and is highly conserved in rat and mouse integrin beta4.
  • Tested applications
    Suitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide conjugated to KLH, corresponding to amino acid residues around tyrosine 1526 of human integrin-beta4.

  • Positive control
    • A431 and src-transformed Hct116 cells treated with pervanadate.
  • General notes


    The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 50% Glycerol, PBS, 1mg/ml BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was cross-adsorbed to phospho-tyrosine coupled to agarose and to dephosphorylated integrin beta4 (Tyr-1526) peptide before affinity purification using phospho-integrin beta-4(Tyr-1526) peptide (without carrier).
  • Primary antibody notes
    The cytoplasmic domain of the integrin-ß4 subunit recruits the adaptor protein Shc and is required for assembly of hemidesmosomes. Tyrosine phosphorylation of multiple sites within the cytoplasmic domain regulates these cellular events. In particular, tyrosine 1526 interacts with the phosphotyrosine binding domain of Shc and is required for Shc activation. In addition, tyrosine 1494 is required for integrin-mediated IRS-2 phosphorylation and activation of PI3-kinase. More importantly, this site is critical for integrin a6ß4 increases in carcinoma invasion.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab29044 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000.
WB 1/1000. Detects a band of approximately 202 kDa.

Target

  • Function
    Integrin alpha-6/beta-4 is a receptor for laminin. It plays a critical structural role in the hemidesmosome of epithelial cells.
  • Tissue specificity
    Integrin alpha-6/beta-4 is predominantly expressed by epithelia. Isoform beta-4D is also expressed in colon and placenta. Isoform beta-4E is also expressed in epidermis, lung, duodenum, heart, spleen and stomach.
  • Involvement in disease
    Defects in ITGB4 are a cause of epidermolysis bullosa letalis with pyloric atresia (EB-PA) [MIM:226730]; also known as junctional epidermolysis bullosa with pyloric atresia (PA-JEB) or aplasia cutis congenita with gastrointestinal atresia. EB-PA is an autosomal recessive, frequently lethal, epidermolysis bullosa with variable involvement of skin, nails, mucosa, and with variable effects on the digestive system. It is characterized by mucocutaneous fragility, aplasia cutis congenita, and gastrointestinal atresia, which most commonly affects the pylorus. Pyloric atresia is a primary manifestation rather than a scarring process secondary to epidermolysis bullosa.
    Defects in ITGB4 are a cause of generalized atrophic benign epidermolysis bullosa (GABEB) [MIM:226650]. GABEB is a non-lethal, adult form of junctional epidermolysis bullosa characterized by life-long blistering of the skin, associated with hair and tooth abnormalities.
  • Sequence similarities
    Belongs to the integrin beta chain family.
    Contains 1 Calx-beta domain.
    Contains 4 fibronectin type-III domains.
    Contains 1 PSI domain.
    Contains 1 VWFA domain.
  • Domain
    The fibronectin type-III-like domains bind BPAG1 and plectin and probably also recruit BP230.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 104 antibody
    • CD104 antibody
    • CD104 antigen antibody
    • gp150 antibody
    • Integrin beta 4 subunit antibody
    • Integrin beta-4 antibody
    • ITB4_HUMAN antibody
    • ITG B4 antibody
    • ITGB 4 antibody
    • Itgb4 antibody
    see all

Anti-Integrin beta 4 (phospho Y1526) antibody images

  • All lanes : Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)

    Lane 1 : A431 cell lysate, starved
    Lane 2 : A431 cell lysate, treated with pervanadate for 30 min

    The top band in each lane is 200 kDa.

References for Anti-Integrin beta 4 (phospho Y1526) antibody (ab29044)

This product has been referenced in:
  • Pickl M & Ries CH Comparison of 3D and 2D tumor models reveals enhanced HER2 activation in 3D associated with an increased response to trastuzumab. Oncogene 28:461-8 (2009). WB ; Human . Read more (PubMed: 18978815) »

See 1 Publication for this product

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