• Product nameAnti-Integrin beta 5 antibody
    See all Integrin beta 5 primary antibodies
  • Description
    Rabbit polyclonal to Integrin beta 5
  • Tested applicationsSuitable for: ICC/IF, IHC-Fr, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control
    • Small intestine, bone marrow and colon carcinoma HeLa Cells Rat gastric epithelial


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.6
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab15459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/20.
IHC-Fr Use at an assay dependent concentration. PubMed: 22025551
IP Use at an assay dependent concentration. PubMed: 22025551
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


Anti-Integrin beta 5 antibody images

  • Human small intestine (ab29276) stained with anti-Integrin beta 5 antibody, ab15459 by Immunohistochemistry (FFPE-sections).

  • ICC/IF image of ab15459 stained HeLa cells (ab150035ab15459), 1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide (ab14083).

    See Abreview

References for Anti-Integrin beta 5 antibody (ab15459)

This product has been referenced in:
  • Sarrazy V  et al. Integrins avß5 and avß3 promote latent TGF-ß1 activation by human cardiac fibroblast contraction. Cardiovasc Res 102:407-17 (2014). Read more (PubMed: 24639195) »
  • Klingberg F  et al. Prestress in the extracellular matrix sensitizes latent TGF-ß1 for activation. J Cell Biol 207:283-97 (2014). ICC/IF ; Human . Read more (PubMed: 25332161) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (Glioma)
Gel Running Conditions Reduced Denaturing (10% gel)
Loading amount 25 µg
Specification Glioma
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted May 12 2015

Unfortunately, this antibody is not against the extracellular domain and so will not be suitable for your application.

We have not tested any of our antibodies in vivo and therefore cannot guarantee that any of these will work on live unpermea...

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Vielen Dank für Ihre Anfrage.

Ich habe Ihnen unser Subcellular Fractionation Protokoll an diese Email gehängt, mit dem Sie eine Isolierung der Zellmembran durchführen können.

Desweiteren habe ich Ihnen unseren &...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (gastric epithelial cell)
Specification gastric epithelial cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton
Blocking step DAKO serum free protein blocker as blocking agent for 20 minute(s) · Concentration: 0.25% · Temperature: 28°C

Dr. J Chai

Verified customer

Submitted Apr 14 2012

Thank you for your inquiry.

Yes, the blocking buffer that was used to generate that image was a combination of 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilize the cells and block non-specific protei...

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Thank you for contacting us. The blocking step requires you to choose either 1%BSA or 10% normal serum for blocking. Incubation should be either for one hour at room temperature or overnight at 4 degrees.

I hope this information is helpfu...

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Thank you for bringing this to our attention. Can you please send the order numbers for each antibody that has discrepancy between the delivered datasheet and the online datasheet? Please also send a brief description of the issues you are having, if a...

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Thank you for contacting us. We would guarantee this antibody to work in both human and mouse tissue sections.  If you had any difficulty in IHC-P with these two species, we would be able to replace or refund the products for you within 6 months of you...

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Thank you for contacting us. The antibody ab15459 has not yet been tested in flow cytometry. If you like to try that, we have a testing discount program you can take advantage of. For UNTESTED species and/or applications, we have estab...

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Thank you for contacting us. The C-terminal region is located in the cytoplasm. Depending on your studies, a permeabilization step would be necessary. I hope this information is helpful to you. Please do not hesitate to contact us if you need any m...

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