For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Synthetic peptide. This information is considered to be commercially sensitive.
Our Abpromise guarantee covers the use of ab15459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22025551|
|IP||Use at an assay dependent concentration. PubMed: 22025551|
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
ICC/IF image of ab15459 stained HeLa cells (ab150035ab15459), 1µg/ml) overnight at +4°C. The secondary antibody (green) was a goat anti-rabbit Alexa Fluor® 488 (IgG; H+L) ab150077) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab15459 staining Integrin beta 5 in Rat gastric epithelial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton and blocked with 0.25% serum free protein for 20 minutes at 28°C. Samples were incubated with primary antibody (1/50 in antibody diluent) for 2 hours at 28°C. An FITC-conjugated Goat anti-IgG polyclonal (1/80) was used as the secondary antibody. Nuclei were counterstained with propidium iodide (ab14083).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"